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Literature summary extracted from

  • Ator, M.A.; Schmidt, S.J.; Adams, J.L.; Dolle, R.E.
    Mechanism and inhibition of delta 24-sterol methyltransferase from Candida albicans and Candida tropicalis (1989), Biochemistry, 28, 9633-9640.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.1.1.41 no stabilization by MgCl2, CaCl2, DTT, reduced glutathione, phosphatidylcholine Candida albicans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.41 desmosterol competitive versus sterol and S-adenosyl-L-methionine Candida albicans
2.1.1.41 desmosterol competitive versus sterol and S-adenosyl-L-methionine Candida tropicalis
2.1.1.41 S-adenosyl-L-homocysteine
-
 Candida albicans
2.1.1.41 Substrate analogues dead-end inhibitors Candida albicans
2.1.1.41 Zymosterol competitive versus sterol and S-adenosyl-L-methionine Candida albicans
2.1.1.41 Zymosterol competitive versus sterol and S-adenosyl-L-methionine Candida tropicalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.41 0.052
-
 Zymosterol
-
 Candida tropicalis
2.1.1.41 0.071
-
 Zymosterol
-
 Candida albicans
2.1.1.41 0.236
-
 desmosterol
-
 Candida tropicalis
2.1.1.41 0.281
-
 desmosterol
-
 Candida albicans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.41 microsome
-
 Candida albicans
-
-
2.1.1.41 microsome
-
 Candida tropicalis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.41 150000
-
 gel filtration Candida albicans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.41 zymosterol + S-adenosyl-L-methionine Candida albicans
-
 fecosterol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.41 zymosterol + S-adenosyl-L-methionine Candida tropicalis
-
 fecosterol + S-adenosyl-L-homocysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.41 Candida albicans
-
-
-
2.1.1.41 Candida tropicalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.41
-
 Candida albicans
2.1.1.41
-
 Candida tropicalis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.41 S-adenosyl-L-methionine + 5alpha-cholesta-8,24-dien-3beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5alpha-cholest-8-en-3beta-ol mechanism Candida albicans
a

Storage Stability

EC Number Storage Stability Organism
2.1.1.41 -70°C, 1 month, less than 10% loss of activity Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.41 desmosterol + S-adenosyl-L-methionine
-
 Candida albicans 24-methylenedesmosterol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.41 desmosterol + S-adenosyl-L-methionine
-
 Candida tropicalis 24-methylenedesmosterol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.41 lanosterol + S-adenosyl-L-methionine no reaction with Candida albicans 24-methylene-24,25-dihydrolanosterol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.41 zymosterol + S-adenosyl-L-methionine
-
 Candida albicans fecosterol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.41 zymosterol + S-adenosyl-L-methionine
-
 Candida tropicalis fecosterol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.41 zymosterol + S-adenosyl-L-methionine zymosterol is 5alpha-cholesta-8,24-dien-3beta-ol Candida albicans fecosterol + S-adenosyl-L-homocysteine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.41 30
-
 assay at Candida albicans
2.1.1.41 30
-
 assay at Candida tropicalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.41 7.5
-
 assay at Candida albicans
2.1.1.41 7.5
-
 assay at Candida tropicalis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.41 0.0045
-
 Zymosterol versus S-adenosyl-L-methionine Candida tropicalis
2.1.1.41 0.009
-
 desmosterol versus S-adenosyl-L-methionine Candida tropicalis
2.1.1.41 0.0193
-
 desmosterol versus S-adenosyl-L-methionine Candida albicans
2.1.1.41 0.022
-
 Zymosterol versus sterol Candida tropicalis
2.1.1.41 0.449
-
 desmosterol versus sterol Candida tropicalis
2.1.1.41 0.489
-
 desmosterol versus sterol Candida albicans