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Literature summary extracted from

  • Nes, W.D.; Janssen, G.G.; Bergenstrahle, A.
    Structural requirements for transformation of substrates by the (S)-adenosyl-L-methionine:delta 24(25)-sterol methyl transferase (1991), J. Biol. Chem., 266, 15202-15212.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.142 additional information no cofactor requirement Helianthus annuus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.142 0.028
-
 lanosterol
-
 Helianthus annuus
2.1.1.142 0.032
-
 Cycloartenol
-
 Helianthus annuus
2.1.1.142 0.033
-
 14alpha-methylzymosterol
-
 Helianthus annuus
2.1.1.142 0.036
-
 31-norcycloartenol
-
 Helianthus annuus
2.1.1.142 0.043
-
 24,25-Dehydropollinastanol
-
 Helianthus annuus
2.1.1.142 0.063
-
 Zymosterol
-
 Helianthus annuus
2.1.1.142 0.067
-
 desmosterol lanosta-7,24-dienol Helianthus annuus
2.1.1.142 0.125
-
 Cholesta-5,7,24-trienol
-
 Helianthus annuus
2.1.1.142 0.13
-
 Agnosterol
-
 Helianthus annuus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.142 microsome
-
 Helianthus annuus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.142 additional information no metal ion required Helianthus annuus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.142 zymosterol + S-adenosyl-L-methionine Helianthus annuus
-
 fecosterol + S-adenosyl-L-homocysteine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.142 Helianthus annuus
-
 sunflower
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.142 S-adenosyl-L-methionine + 5alpha-cholesta-8,24-dien-3beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5alpha-cholest-8-en-3beta-ol mechanism Helianthus annuus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.142 embryo
-
 Helianthus annuus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.142 14alpha-methylzymosterol + S-adenosyl-L-methionine 14alpha-methylzymosterol is 14alpha-methylcholesta-8,24-dien-3beta-ol Helianthus annuus 14alpha-methyl-24-methylenezymosterol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.142 24,25-dehydropollinastanol + S-adenosyl-L-methionine 24,25-dehydropollinastanol is 14alpha-methyl-9beta,19-cyclo-5alpha-cholest-24-en-3beta-ol Helianthus annuus ?
-
 ?
2.1.1.142 31-norcycloartenol + S-adenosyl-L-methionine 31-norcycloartenol is 4,14alpha-dimethyl-9beta,19-cyclo-5alpha-cholest-24-en-3beta-ol Helianthus annuus 24-methylene-31-norcycloartenol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.142 agnosterol + S-adenosyl-L-methionine agnosterol is 4,4,14alpha-trimethyl-5alpha-cholesta-7(8),9(11),24-trien-3beta-ol Helianthus annuus 24-methyleneagnosterol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.142 cholesta-5,7,24-trienol + S-adenosyl-L-methionine
-
 Helianthus annuus ?
-
 ?
2.1.1.142 cycloartenol + S-adenosyl-L-methionine cycloartenol is 4,4,14alpha-trimethyl-9beta,19-cyclo-5alpha-cholest-24-en-3beta-ol Helianthus annuus 24-methylenecycloartanol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.142 cycloartenol 3-ketone + S-adenosyl-L-methionine cycloartenol 3-ketone is 4,4,14alpha-trimethyl-9beta,19-cyclo-5alpha-cholest-24-en-3-one Helianthus annuus ?
-
 ?
2.1.1.142 desmosterol + S-adenosyl-L-methionine desmosterol is 5alpha-cholesta-5,24-dien-3beta-ol Helianthus annuus 24-methylenedesmosterol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.142 lanost-24-enol + S-adenosyl-L-methionine lanost-24-enol is 4,4,14alpha-trimethyl-5alpha-cholesta-9(11),24-dien-3beta-ol Helianthus annuus 24-methylene-lanost-24-erol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.142 lanosta-7,24-dienol + S-adenosyl-L-methionine lanosta-7,24-dienol is 4,4,14alpha-trimethyl-5alpha-cholesta-7(8),24-dien-3beta-ol Helianthus annuus 24-methylene-lanost-7,24-dienol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.142 lanosterol + S-adenosyl-L-methionine lanosterol is 4,4,14alpha-trimethyl-5alpha-cholesta-8,24-dien-3-ol Helianthus annuus 24-methylene-24,25-dihydrolanosterol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.142 zymosterol + S-adenosyl-L-methionine
-
 Helianthus annuus fecosterol + S-adenosyl-L-homocysteine
-
 ?
2.1.1.142 zymosterol + S-adenosyl-L-methionine zymosterol is 5alpha-cholesta-8,24-dien-3beta-ol Helianthus annuus fecosterol + S-adenosyl-L-homocysteine
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.142 7.5
-
-
 Helianthus annuus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.142 5.5 10 slightly active at pH 5.5, no activity at pH 10 Helianthus annuus