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Literature summary extracted from

  • Koller, F.; Hoffmann-Ostenhof, O.
    Studies on the biosynthesis of cyclitols, XXXIV[l] Purification of myo-inositol 3-methyltransferase from Pisum sativum and of myo-inositol 1-methyltransferase from Vinca minor to homogeneity by affinity chromatography (1976), Hoppe-Seyler's Z. Physiol. Chem., 357, 1465-1468.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.39 0.8
-
 S-adenosyl-L-methionine
-
 Vinca minor
2.1.1.39 2.5
-
 myo-inositol
-
 Vinca minor
2.1.1.40 2.5
-
 S-adenosyl-L-methionine
-
 Pisum sativum
2.1.1.40 2.9
-
 myo-inositol
-
 Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.39 27000
-
 gel filtration Vinca minor
2.1.1.40 42000
-
 gel filtration, PAGE Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.39 S-adenosyl-L-methionine + myo-inositol Vinca minor
-
 S-adenosyl-L-homocysteine + 1D-3-O-methyl-myo-inositol + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.39 Vinca minor
-
-
-
2.1.1.40 Pisum sativum
-
 pea
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.39
-
 Vinca minor
2.1.1.40
-
 Pisum sativum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.39 0.0078
-
-
 Vinca minor
2.1.1.40 0.0078
-
 kat/g Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.39 S-adenosyl-L-methionine + myo-inositol
-
 Vinca minor S-adenosyl-L-homocysteine + 1D-3-O-methyl-myo-inositol + H+
-
 ?
2.1.1.39 S-adenosyl-L-methionine + myo-inositol
-
 Vinca minor S-adenosyl-L-homocysteine + 1D-3-O-methyl-myo-inositol + H+ i.e. pinitol ?
2.1.1.40 S-adenosyl-L-methionine + myo-inositol
-
 Pisum sativum S-adenosyl-L-homocysteine + D-bornesitol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.39 monomer 1 * 27000, SDS-PAGE Vinca minor
2.1.1.40 monomer 1 * 42000, SDS-PAGE Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.39 7
-
-
 Vinca minor
2.1.1.40 7
-
-
 Pisum sativum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.39 Vinca minor isoelectric focusing
-
 7.1