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Literature summary extracted from

  • Aoki, A.; Suetake, I.; Miyagawa, J.; Fujio, T.; Chijiwa, T.; Sasaki, H.; Tajima, S.
    Enzymatic properties of de novo-type mouse DNA (cytosine-5) methyltransferases (2001), Nucleic Acids Res., 29, 3506-3512.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.37 KCl above 100 mM Mus musculus
2.1.1.37 NaCl above 100 mM Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.37 0.0003
-
 poly(dI-dC)-poly(dI-dC) isoenzyme Dnmt3a Mus musculus
2.1.1.37 0.0003
-
 S-adenosyl-L-methionine isoenzyme Dnmt3a, reaction with poly(dG-dC)-poly(dG-dC) Mus musculus
2.1.1.37 0.0004
-
 S-adenosyl-L-methionine isoenzyme Dnmt3a, reaction with poly(dI-dC)-poly(dI-dC) Mus musculus
2.1.1.37 0.0008
-
 S-adenosyl-L-methionine isoenzyme Dnmt3b2, reaction with poly(dG-dC)-poly(dG-dC) Mus musculus
2.1.1.37 0.0009
-
 S-adenosyl-L-methionine isoenzyme Dnmt3b2, reaction with poly(dI-dC)-poly(dI-dC) Mus musculus
2.1.1.37 0.0009
-
 poly(dG-dC)-poly(dG-dC) isoenzyme Dnmt3b2 Mus musculus
2.1.1.37 0.001
-
 poly(dG-dC)-poly(dG-dC) isoenzyme Dnmt3b1 Mus musculus
2.1.1.37 0.0012
-
 S-adenosyl-L-methionine isoenzyme Dnmt3b1, reaction with poly(dI-dC)-poly(dI-dC) por poly(dG-dC)*poly(dG-dC) Mus musculus
2.1.1.37 0.0013
-
 poly(dI-dC)-poly(dI-dC) isoenzyme Dnmt3b1 Mus musculus
2.1.1.37 0.0015
-
 poly(dI-dC)-poly(dI-dC) isoenzyme Dnmt3b2 Mus musculus
2.1.1.37 0.0027
-
 poly(dG-dC)-poly(dG-dC) isoenzyme Dnmt3a Mus musculus
2.1.1.37 0.0035
-
 poly(dG-dC)-poly(dG-dC) isoenzyme Dnmt3a Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.37 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.37 recombinant Dnmt3a, Dnmt3b1 and Dnmt3b2, GST-fused Dnmt3 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.37 S-adenosyl-L-methionine + DNA
-
 Mus musculus S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?
2.1.1.37 S-adenosyl-L-methionine + DNA isoenzymes Dnmt3a, Dnmt3b1 and Dnmt3b2 show similar activity towards poly(dG-dC)-poly(dG-dC) Mus musculus S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?
2.1.1.37 S-adenosyl-L-methionine + poly(dG-dC)-poly(dG-dC)
-
 Mus musculus ?
-
 ?
2.1.1.37 S-adenosyl-L-methionine + poly(dI-dC)-poly(dI-dC)
-
 Mus musculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.37 More the apparent molecular weights of the GST-fused Dnmt3a, Dnmt3b1 and Dnmt3b2 are 130000 Da, 120000 Da and 115000 Da, as determined by SDS-PAGE Mus musculus