EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.37 | construction of three hybrid methyltransferases, containing the intact N-terminus of the murine Dnmt1 and most of the coding sequences from M.HhaI (GCGC), M.HpaII (CCGG) or M.SssI (CG). The hybrids are biologically active when expressed in a baculovirus system and show the specificity of the parental C-terminal domain | Mus musculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.37 | additional information | - |
additional information | Km-values for hybrid enzymes | Mus musculus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.37 | 170000 | - |
hybrid enzymes Dnmt1-HhaI and Dnmt1-HpaII | Mus musculus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.37 | Mus musculus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.37 | Dnmt1-HhaI and Dnmt1-HpaII hybrid enzymes | Mus musculus |
EC Number | Storage Stability | Organism |
---|---|---|
2.1.1.37 | -20°C, hybrid enzymes Dnmt1-HhaI and Dnmt1-HpaII are stable for several weeks | Mus musculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.37 | S-adenosyl-L-methionine + DNA | - |
Mus musculus | S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.37 | additional information | - |
additional information | turnover-numbers for hybrid enzymes | Mus musculus |