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Literature summary extracted from

  • Laayoun, A.; Smith, S.S.
    Methylation of slipped duplexes, snapbacks and cruciforms by human DNA(cytosine-5)methyltransferase (1995), Nucleic Acids Res., 23, 1584-1589.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.37 Homo sapiens
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.37 placenta
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 Homo sapiens
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.37 S-adenosyl-L-methionine + DNA
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 Homo sapiens S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
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 ?
2.1.1.37 S-adenosyl-L-methionine + DNA methylation of a series of snapback oligodeoxynucleotides of differing stem length, each containing a centrally located CG dinucleotide recognition site. The enzyme requires a minimum of 22 base pairs in the stem for maximum activity. Extrahelical cytosines in slipped duplexes that are 30 base pairs in length act as effective methyl acceptors and are more rapidly methylated than cytosines that are Watson-Crick paired. Duplexes containing hairpins of CCG repeats in cruciform structures in which the enzyme recognition sequence is disrupted by a C-C mispair are also more rapidly methylated than the control Watson-Crick-paired duplexes Homo sapiens S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
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 ?