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Literature summary extracted from

  • Brank, A.S.; Van Bemmel, D.M.; Christman, J.K.
    Optimization of baculovirus-mediated expression and purification of hexahistidine-tagged murine DNA (cytosine-C5)-methyltransferase-1 in Spodoptera frugiperda 9 cells (2002), Protein Expr. Purif., 25, 31-40.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.37 DNA (cytosine C5) methyltransferase-1, fused to an amino-terminal hexahistidine tag, is expressed by infecting Spodoptera frugiperda cells for 46 h with a recombinant baculovirus carrying the DNA (cytosine-C5)methyltransferase-1 cDNA Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.37 additional information
-
 additional information
-
 Mus musculus
2.1.1.37 0.000973
-
 poly(dIdC:dIdC)
-
 Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.37 190000
-
 x * 190000, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.37 additional information Mus musculus enzymatic DNA methylation of carbon 5 of cytosines is an epigenetic modification that plays a role in regulating gene expression, differentiation, and tumorigenesis. DNA (cytosine-C5)-methyltransferase-1 is the enzyme responsible for maintaining established methylation patterns during replication in mammalian cells ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.37 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.37
-
 Mus musculus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.37 additional information
-
-
 Mus musculus

Storage Stability

EC Number Storage Stability Organism
2.1.1.37 -20°C, hexahistidine-tagged enzyme is stable for several months Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.37 additional information enzymatic DNA methylation of carbon 5 of cytosines is an epigenetic modification that plays a role in regulating gene expression, differentiation, and tumorigenesis. DNA (cytosine-C5)-methyltransferase-1 is the enzyme responsible for maintaining established methylation patterns during replication in mammalian cells Mus musculus ?
-
 ?
2.1.1.37 S-adenosyl-L-methionine + DNA
-
 Mus musculus S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?
2.1.1.37 S-adenosyl-L-methionine + DNA the purified enzyme has at least a 30fold higher catalytic efficiency with hemimethylated double-stranded oligodeoxyribonucleotide substrates than unmethylated substrates and is most active with small oligodeoxyribonucleotide substrates Mus musculus S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?
2.1.1.37 S-adenosyl-L-methionine + DNA poly(dIdC:dIdC) is efficiently methylated Mus musculus S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?
2.1.1.37 S-adenosyl-L-methionine + poly(dIdC:dIdC)
-
 Mus musculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.37 ? x * 190000, SDS-PAGE Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.37 additional information
-
 additional information
-
 Mus musculus
2.1.1.37 0.0061
-
 poly(dIdC:dIdC)
-
 Mus musculus