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Literature summary extracted from

  • Theiss, G.; Schleicher, R.; Schimpff-Weiland, G.; Follmann, H.
    DNA methylation in wheat. Purification and properties of DNA methyltransferase (1987), Eur. J. Biochem., 167, 89-96.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.37 Ca2+ above 1 mM Triticum aestivum
2.1.1.37 KCl above 0.1 M, inhibition may be simply due to dissociation of the DNA-enzyme complex Triticum aestivum
2.1.1.37 NaCl above 0.1 M, inhibition may be simply due to dissociation of the DNA-enzyme complex Triticum aestivum
2.1.1.37 S-adenosyl-L-ethionine IC50: 0.05 mM Triticum aestivum
2.1.1.37 S-adenosyl-L-homocysteine half-inhibition at 0.02 mM Triticum aestivum
2.1.1.37 spermidine at millimolar concentrations Triticum aestivum
2.1.1.37 spermine at millimolar concentrations Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.37 0.005 0.006 S-adenosyl-L-methionine
-
 Triticum aestivum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.37 nucleus
-
 Triticum aestivum 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.37 50000 55000 gel filtration, sucrose density gradient centrifugation Triticum aestivum

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.37 Triticum aestivum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.37
-
 Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.37 embryo germinating embryo Triticum aestivum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.37 additional information
-
-
 Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.37 S-adenosyl-L-methionine + DNA
-
 Triticum aestivum S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?
2.1.1.37 S-adenosyl-L-methionine + DNA methylation of cytosine residues in double-stranded DNAs isolated from wheat, maize, calf thymus or bacteria. Single-stranded DNAs from all sources are methylated to only about one-fourth to one-third the degree of double-stranded substrate DNA. Synthetic polydeoxyribonucleotides of alternating base sequence, poly[d(G-C)]*poly[d(G-C)], poly[d(I-C)]*poly[d(I-C)], poly[d(A-C)]*poly[d(G-T)], are methylated to low but clearly measurable extent. Preference for endogenous, double-stranded DNA Triticum aestivum S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.37 7.5 8
-
 Triticum aestivum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.37 6 9 about 50% of maximal activity at pH 6 and pH 9 Triticum aestivum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.1.1.37 0.05
-
 IC50: 0.05 mM Triticum aestivum S-adenosyl-L-ethionine