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Literature summary extracted from

  • Sano, H.; Noguchi, H.; Sager, R.
    Characterization of DNA methyltransferase from bovine thymus cells (1983), Eur. J. Biochem., 135, 181-185.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.37 native DNA non-competitive inhibitor against S-adenosyl-L-methionine Bos taurus
2.1.1.37 S-adenosyl-L-ethionine non-competitive inhibitor against DNA Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.37 130000
-
 glycerol density gradient centrifugation Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.37 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.37 partial Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.37 S-adenosyl-L-methionine + DNA containing cytosine = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine random sequential mechanism Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.37 thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.37 additional information
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.1.1.37 -20°C, enzyme loses activity within several weeks Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.37 S-adenosyl-L-methionine + DNA
-
 Bos taurus S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?
2.1.1.37 S-adenosyl-L-methionine + DNA about 60% of the total methylation occurs in the 5'd(C-G)3' doublet. Single-stranded and hemi-methylated DNAs are methylated at an elevated rate Bos taurus S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?