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Literature summary extracted from

  • Giordano, M.; Mattachini, M.E.; Cella, R.; Pedrali-Noy, G.
    Purification and properties of a novel DNA methyltransferase from cultured rice cells (1991), Biochem. Biophys. Res. Commun., 177, 711-719.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.37 additional information
-
 additional information Km-value for poly(dI-MedC)*poly(dI-dC): 0.017 mg/ml Oryza sativa
2.1.1.37 0.0026
-
 S-adenosyl-L-methionine
-
 Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.37 54000
-
 sucrose density gradient centrifugation Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.37 Oryza sativa
-
 L. cv Roncarolo
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.37 cell suspension culture
-
 Oryza sativa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.37 additional information
-
-
 Oryza sativa

Storage Stability

EC Number Storage Stability Organism
2.1.1.37 -20°C, stable for more than 2 months Oryza sativa
2.1.1.37 4°C, stable for 5 days Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.37 S-adenosyl-L-methionine + DNA
-
 Oryza sativa S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?
2.1.1.37 S-adenosyl-L-methionine + DNA native plant DNA or hemimethylated poly(dI-MedC)*poly(dI-dC) Oryza sativa S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.37 4
-
 stable for 5 days Oryza sativa
2.1.1.37 30
-
 15 min, stable Oryza sativa
2.1.1.37 42
-
 half-life: 48 s Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.37 8
-
-
 Oryza sativa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.37 7.6 8.7 50% of maximal activity at pH 7.6 and at pH 8.7 Oryza sativa