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Literature summary extracted from

  • Hersh, L.B.
    N-Methyl-L-glutamate synthase (1985), Methods Enzymol., 113, 36-42.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.21 30000 35000 SDS-PAGE Pseudomonas sp.
2.1.1.21 350000 390000 density gradient centrifugation Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.21 methylamine + L-glutamate Pseudomonas sp. involved in one carbon metabolism NH3 + N-methyl-L-glutamate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.21 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.21
-
Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.21 0.138
-
purified enzyme Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.21 butylamine + L-glutamate at 5% the rate of the reaction with methylamine Pseudomonas sp. NH3 + N-butyl-L-glutamate
-
?
2.1.1.21 ethanolamine + L-glutamate at 18% the rate of the reaction with methylamine Pseudomonas sp. ?
-
?
2.1.1.21 ethylamine + L-glutamate at 16% the rate of reaction with methylamine Pseudomonas sp. NH3 + N-ethyl-L-glutamate
-
?
2.1.1.21 methylamine + L-glutamate
-
Pseudomonas sp. NH3 + N-methyl-L-glutamate
-
r
2.1.1.21 methylamine + L-glutamate involved in one carbon metabolism Pseudomonas sp. NH3 + N-methyl-L-glutamate
-
r
2.1.1.21 N-methyl-L-glutamate + ammonia at 54% the rate of the reaction with cysteamine Pseudomonas sp. ?
-
?
2.1.1.21 N-methyl-L-glutamate + butylamine at 7% the rate of the reaction with ammonia Pseudomonas sp. ?
-
?
2.1.1.21 N-methyl-L-glutamate + ethanolamine at 27% the rate of the reaction with ammonia Pseudomonas sp. ?
-
?
2.1.1.21 N-methyl-L-glutamate + ethylamine at 32% the rate of the reaction with ammonia Pseudomonas sp. ?
-
?
2.1.1.21 N-methyl-L-glutamate + methylamine at 70% the rate of the reaction with ammonia Pseudomonas sp. ?
-
?
2.1.1.21 N-methyl-L-glutamate + propylamine at 18% the rate of the reaction with ammonia Pseudomonas sp. ?
-
?
2.1.1.21 propylamine + L-glutamate at 8% the rate of the reaction with methylamine Pseudomonas sp. NH3 + N-propyl-L-glutamate
-
?

Subunits

EC Number Subunits Comment Organism
2.1.1.21 multimer SDS-PAGE, density gradient centrifugation Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.21 9
-
-
Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.21 FMN
-
Pseudomonas sp.