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Literature summary extracted from

  • Hamahata, A.; Takata, Y.; Gomi, T.; Fujioka, M.
    Probing the S-adenosylmethionine-binding site of rat guanidinoacetate methyltransferase: effect of site-directed mutagenesis of residues that are conserved across mammalian non-nucleic acid methyltransferases (1996), Biochem. J., 317, 141-145.
No PubMed abstract available

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.2 D92N lower activity than wild type enzyme Rattus norvegicus
2.1.1.2 E89Q lower activity than wild type enzyme Rattus norvegicus
2.1.1.2 G67A inactive enzyme Rattus norvegicus
2.1.1.2 G69A inactive enzyme Rattus norvegicus
2.1.1.2 W143F lower activity than wild type enzyme Rattus norvegicus
2.1.1.2 W143L lower activity than wild type enzyme Rattus norvegicus
2.1.1.2 Y133V lower activity than wild type enzyme Rattus norvegicus
2.1.1.2 Y136F higher turnover than wild type enzyme Rattus norvegicus
2.1.1.2 Y136V lower activity than wild type enzyme Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.2 additional information
-
 additional information all mutant enzymes listed with Km Rattus norvegicus
2.1.1.2 0.005
-
 S-adenosyl-L-methionine wild type enzyme Rattus norvegicus
2.1.1.2 0.011
-
 Guanidinoacetate wild type enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.2 S-adenosyl-L-methionine + guanidinoacetate Rattus norvegicus
-
 S-adenosyl-L-homocysteine + creatine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.2 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.2
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.2 S-adenosyl-L-methionine + guanidinoacetate
-
 Rattus norvegicus S-adenosyl-L-homocysteine + creatine
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.2 additional information
-
 additional information all mutant enzymes listed with turnover Rattus norvegicus
2.1.1.2 0.0788
-
 Guanidinoacetate wild type enzyme Rattus norvegicus