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Literature summary extracted from

  • Ogawa, H.; Date, T.; Gomi, T.; Konishi, K.; Pitot, H.C.; Cantoni, G.L.; Fujioka, M.
    Molecular cloning, sequence analysis, and expression in Escherichia coli of the cDNA for guanidinoacetate methyltransferase from rat liver (1988), Proc. Natl. Acad. Sci. USA, 85, 694-698.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.2 in Escherichia coli Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.2 0.003
-
 S-adenosyl-L-methionine native enzyme Rattus norvegicus
2.1.1.2 0.003
-
 S-adenosyl-L-methionine recombinant enzyme Rattus norvegicus
2.1.1.2 0.031
-
 Guanidinoacetate native enzyme Rattus norvegicus
2.1.1.2 0.032
-
 Guanidinoacetate recombinant enzyme Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.2 26000
-
 SDS-PAGE, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.2 S-adenosyl-L-methionine + guanidinoacetate Rattus norvegicus
-
 S-adenosyl-L-homocysteine + creatine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.2 Rattus norvegicus
-
 expressed in Escherichia coli
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.2 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.2 0.12
-
 native enzyme Rattus norvegicus
2.1.1.2 0.13
-
 recombinant enzyme Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.2 S-adenosyl-L-methionine + guanidinoacetate
-
 Rattus norvegicus S-adenosyl-L-homocysteine + creatine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.2 monomer SDS-PAGE, gel filtration Rattus norvegicus