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Literature summary extracted from

  • Wang, J.; Pichersky, E.
    Characterization of S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase involved in floral scent production in Clarkia breweri (1998), Arch. Biochem. Biophys., 349, 153-160.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.146 expression in Escherichia coli Clarkia breweri

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.146 Ca2+ 5 mM, weak inhibition Clarkia breweri
2.1.1.146 Cu2+ 5 mM, strong inhibition Clarkia breweri
2.1.1.146 Fe2+ 5 mM, strong inhibition Clarkia breweri
2.1.1.146 Fe3+ 5 mM, strong inhibition Clarkia breweri
2.1.1.146 K+ 5 mM, weak inhibition Clarkia breweri
2.1.1.146 Mg2+ 5 mM, 33% inhibition Clarkia breweri
2.1.1.146 Mn2+ 5 mM, strong inhibition Clarkia breweri
2.1.1.146 Na+ 5 mM, weak inhibition Clarkia breweri
2.1.1.146 NH4+ 5 mM, weak inhibition Clarkia breweri
2.1.1.146 Zn2+ 5 mM, strong inhibition Clarkia breweri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.146 0.007
-
 eugenol plant-purified enzyme Clarkia breweri
2.1.1.146 0.019
-
 S-adenosyl-L-methionine recombinant enzyme Clarkia breweri
2.1.1.146 0.027
-
 S-adenosyl-L-methionine plant-purified enzyme Clarkia breweri
2.1.1.146 0.03
-
 eugenol recombinant enzyme Clarkia breweri
2.1.1.146 0.058
-
 isoeugenol plant-purified enzyme Clarkia breweri
2.1.1.146 0.074
-
 isoeugenol recombinant enzyme Clarkia breweri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.146 additional information methylation of eugenol and isoeugenol Clarkia breweri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.146 40000
-
 2 * 40000, SDS-PAGE Clarkia breweri
2.1.1.146 88000
-
 gel filtration Clarkia breweri

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.146 Clarkia breweri O04385
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.146
-
 Clarkia breweri

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.146 flower
-
 Clarkia breweri
-
2.1.1.146 petal
-
 Clarkia breweri
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.146 additional information
-
-
 Clarkia breweri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.146 S-adenosyl-L-methionine + eugenol
-
 Clarkia breweri S-adenosyl-L-homocysteine + methyleugenol
-
 ?
2.1.1.146 S-adenosyl-L-methionine + isoeugenol
-
 Clarkia breweri S-adenosyl-L-homocysteine + isomethyleugenol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.146 dimer 2 * 40000, SDS-PAGE Clarkia breweri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.146 35
-
 30 min, stable up to Clarkia breweri
2.1.1.146 45
-
 30 min, activity is dramatically decreased Clarkia breweri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.146 7.5
-
 methylation of eugenol and isoeugenol Clarkia breweri