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Literature summary extracted from

  • Poon, W.W.; Barkovich, R.J.; Hsu, A.Y.; Frankel, A.; Lee, P.T.; Sheperd, J.N.; Myles, D.C.; Clarke, C.F.
    Yeast and rat Coq3 and Escherichia coli UbiG polypeptides catalyze both O-methyltransferase steps in coenzyme Q biosynthesis (1999), J. Biol. Chem., 274, 21665-21672.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.64 rat COQ3 gene restores O-methyltransferase activity in coq3 null mutant yeast Rattus norvegicus
2.1.1.114 rat COQ3 gene restores O-methyltransferase activity in coq3 null mutant yeast Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.64 mitochondrion the Coq3 polypeptide is peripherally associated with the matrix side of the inner membrane of yeast mitochondria Saccharomyces cerevisiae 5739
-
2.1.1.114 mitochondrion the Coq3 polypeptide is peripherally associated with the matrix side of the inner membrane of yeast mitochondria Saccharomyces cerevisiae 5739
-
2.1.1.222 mitochondrion the Coq3 polypeptide is peripherally associated with the matrix side of the inner membrane of yeast mitochondria Saccharomyces cerevisiae 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.64 S-adenosyl-L-methionine + 3-demethylubiquinol-6 Saccharomyces cerevisiae the enzyme catalyzes two methylation steps in the biosynthesis of ubiquinone-6 in Saccharomyces cerevisiae, 1. the methylation of 3,4-dihydroxy-5-all-trans-hexaprenylbenzoate (this reaction is classified as EC 2.1.1.114) and 2. the methylation of 3-demethylubiquinol-6 S-adenosyl-L-homocysteine + ubiquinol-6
-
 ?
2.1.1.64 S-adenosyl-L-methionine + 3-demethylubiquinol-8 Escherichia coli the enzyme catalyzes two methylation steps in the biosynthesis of ubiquinone-8 in Escherichia coli, 1. the methylation of 3,4-dihydroxy-5-all-trans-octaprenylbenzoate (this reaction is classified as EC 2.1.1.114) and 2. the methylation of 3-demethylubiquinol-8 S-adenosyl-L-homocysteine + ubiquinol-8
-
 ?
2.1.1.64 S-adenosyl-L-methionine + 3-demethylubiquinol-9 Rattus norvegicus the enzyme catalyzes two methylation steps in the biosynthesis of ubiquinone-9 in Rattus norvegicus, 1. the methylation of 3,4-dihydroxy-5-all-trans-octaprenylbenzoate (this reaction is classified as EC 2.1.1.114) and 2. the methylation of 3-demethylubiquinol-9 S-adenosyl-L-homocysteine + ubiquinol-9
-
 ?
2.1.1.114 S-adenosyl-L-methionine + 3,4-dihydroxy-5-hexaprenylbenzoate Saccharomyces cerevisiae the enzyme catalyzes two methylation steps in the biosynthesis of ubiquinone-6, 1. the methylation of 3,4-dihydroxy-5-hexaprenylbenzoate and 2. the methylation of 3-demethylubiquinol-6 (this reaction is classified as EC 2.1.1.64) S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-5-hexaprenylbenzoate
-
 ?
2.1.1.114 S-adenosyl-L-methionine + 3,4-dihydroxy-5-octaprenylbenzoate Rattus norvegicus the enzyme catalyzes two methylation steps in the biosynthesis of ubiquinone-8, 1. the methylation of 3,4-dihydroxy-5-octaprenylbenzoate and 2. the methylation of 3-demethylubiquinol-8 (this reaction is classified as EC 2.1.1.64) S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxy-5-octaprenylbenzoate
-
 ?
2.1.1.222 additional information Saccharomyces cerevisiae the enzyme catalyzes two methylation steps in the biosynthesis of ubiquinone-6 in Saccharomyces cerevisiae, 1. the methylation of 3,4-dihydroxy-5-hexaprenylbenzoate (this reaction is classified as EC 2.1.1.114) and 2. the methylation of 3-demethylubiquinol-6 (this reaction is classified as EC 2.1.1.64) ?
-
 ?
2.1.1.222 additional information Rattus norvegicus the enzyme catalyzes two methylation steps in the biosynthesis of ubiquinone-9 in Rattus norvegicus, 1. the methylation of 3,4-dihydroxy-5-nonaprenylbenzoate (this reaction is classified as EC 2.1.1.114) and 2. the methylation of 3-demethylubiquinol-9 (this reaction is classified as EC 2.1.1.64) ?
-
 ?
2.1.1.222 S-adenosyl-L-methionine + 3-(all-trans-octaprenyl)benzene-1,2-diol Escherichia coli the enzyme catalyzes two methylation steps in the biosynthesis of ubiquinone-8 in Escherichia coli, 1. the methylation of 3-(all-trans-octaprenyl)benzene-1,2-diol and 2. the methylation of 3-demethylubiquinol-8 (this reaction is classified as EC 2.1.1.64) S-adenosyl-L-homocysteine + 2-methoxy-6-(all-trans-octaprenyl)phenol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.64 Escherichia coli
-
-
-
2.1.1.64 Rattus norvegicus Q63159
-
-
2.1.1.64 Saccharomyces cerevisiae P27680
-
-
2.1.1.114 Rattus norvegicus Q63159
-
-
2.1.1.114 Saccharomyces cerevisiae P27680
-
-
2.1.1.222 Escherichia coli
-
-
-
2.1.1.222 Rattus norvegicus Q63159
-
-
2.1.1.222 Saccharomyces cerevisiae P27680
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.222 His6-UbiG fusion protein Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.64 S-adenosyl-L-methionine + 3-demethylubiquinol-3 i.e. 5-(2E,6E)-farnesyl-2-hydroxy-3-methoxy-6-methyl-1,4-benzoquinone Escherichia coli S-adenosyl-L-homocysteine + ubiquinol-3
-
 ?
2.1.1.64 S-adenosyl-L-methionine + 3-demethylubiquinol-3 i.e. 5-(2E,6E)-farnesyl-2-hydroxy-3-methoxy-6-methyl-1,4-benzoquinone Saccharomyces cerevisiae S-adenosyl-L-homocysteine + ubiquinol-3
-
 ?
2.1.1.64 S-adenosyl-L-methionine + 3-demethylubiquinol-3 i.e. 5-(2E,6E)-farnesyl-2-hydroxy-3-methoxy-6-methyl-1,4-benzoquinone, in vitro assay Rattus norvegicus S-adenosyl-L-homocysteine + ubiquinol-3
-
 ?
2.1.1.64 S-adenosyl-L-methionine + 3-demethylubiquinol-6 the enzyme catalyzes two methylation steps in the biosynthesis of ubiquinone-6 in Saccharomyces cerevisiae, 1. the methylation of 3,4-dihydroxy-5-all-trans-hexaprenylbenzoate (this reaction is classified as EC 2.1.1.114) and 2. the methylation of 3-demethylubiquinol-6 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + ubiquinol-6
-
 ?
2.1.1.64 S-adenosyl-L-methionine + 3-demethylubiquinol-8 the enzyme catalyzes two methylation steps in the biosynthesis of ubiquinone-8 in Escherichia coli, 1. the methylation of 3,4-dihydroxy-5-all-trans-octaprenylbenzoate (this reaction is classified as EC 2.1.1.114) and 2. the methylation of 3-demethylubiquinol-8 Escherichia coli S-adenosyl-L-homocysteine + ubiquinol-8
-
 ?
2.1.1.64 S-adenosyl-L-methionine + 3-demethylubiquinol-9 the enzyme catalyzes two methylation steps in the biosynthesis of ubiquinone-9 in Rattus norvegicus, 1. the methylation of 3,4-dihydroxy-5-all-trans-octaprenylbenzoate (this reaction is classified as EC 2.1.1.114) and 2. the methylation of 3-demethylubiquinol-9 Rattus norvegicus S-adenosyl-L-homocysteine + ubiquinol-9
-
 ?
2.1.1.114 S-adenosyl-L-methionine + 3,4-dihydroxy-5-((2E,6E)-farnesyl)benzoate
-
 Rattus norvegicus S-adenosyl-L-homocysteine + 5-((2E,6E)-farnesyl)-4-hydroxy-3-methoxybenzoate
-
 ?
2.1.1.114 S-adenosyl-L-methionine + 3,4-dihydroxy-5-(2E,6E)-farnesylbenzoate
-
 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-5-farnesylbenzoate
-
 ?
2.1.1.114 S-adenosyl-L-methionine + 3,4-dihydroxy-5-hexaprenylbenzoate the enzyme catalyzes two methylation steps in the biosynthesis of ubiquinone-6, 1. the methylation of 3,4-dihydroxy-5-hexaprenylbenzoate and 2. the methylation of 3-demethylubiquinol-6 (this reaction is classified as EC 2.1.1.64) Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-5-hexaprenylbenzoate
-
 ?
2.1.1.114 S-adenosyl-L-methionine + 3,4-dihydroxy-5-octaprenylbenzoate the enzyme catalyzes two methylation steps in the biosynthesis of ubiquinone-8, 1. the methylation of 3,4-dihydroxy-5-octaprenylbenzoate and 2. the methylation of 3-demethylubiquinol-8 (this reaction is classified as EC 2.1.1.64) Rattus norvegicus S-adenosyl-L-homocysteine + 4-hydroxy-3-methoxy-5-octaprenylbenzoate
-
 ?
2.1.1.222 additional information the enzyme catalyzes two methylation steps in the biosynthesis of ubiquinone-6 in Saccharomyces cerevisiae, 1. the methylation of 3,4-dihydroxy-5-hexaprenylbenzoate (this reaction is classified as EC 2.1.1.114) and 2. the methylation of 3-demethylubiquinol-6 (this reaction is classified as EC 2.1.1.64) Saccharomyces cerevisiae ?
-
 ?
2.1.1.222 additional information the enzyme catalyzes two methylation steps in the biosynthesis of ubiquinone-9 in Rattus norvegicus, 1. the methylation of 3,4-dihydroxy-5-nonaprenylbenzoate (this reaction is classified as EC 2.1.1.114) and 2. the methylation of 3-demethylubiquinol-9 (this reaction is classified as EC 2.1.1.64) Rattus norvegicus ?
-
 ?
2.1.1.222 S-adenosyl-L-methionine + 3-((2E,6E)-farnesyl)benzene-1,2-diol i.e. 3-[(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]benzene-1,2-diol Escherichia coli S-adenosyl-L-homocysteine + 2-methoxy-6-((2E,6E)-farnesyl)phenol i.e. 2-methoxy-6-((2E,6E)-3,7,11-trimethyldodeca-2,6,10-trienyl)phenol ?
2.1.1.222 S-adenosyl-L-methionine + 3-((2E,6E)-farnesyl)benzene-1,2-diol i.e. 3-[(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]benzene-1,2-diol. The substrate is an artificial farnesylated analog of the Escherichia coli substrate 2-octaprenyl-6-hydroxyphenol, an intermediate in the biosynthesis of ubiquinone-8 in Escherichia coli Rattus norvegicus S-adenosyl-L-homocysteine + 2-methoxy-6-((2E,6E)-farnesyl)phenol i.e. 2-methoxy-6-((2E,6E)-3,7,11-trimethyldodeca-2,6,10-trienyl)phenol ?
2.1.1.222 S-adenosyl-L-methionine + 3-((2E,6E)-farnesyl)benzene-1,2-diol i.e. 3-[(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]benzene-1,2-diol. The substrate is an artificial farnesylated analog of the Escherichia coli substrate 2-octaprenyl-6-hydroxyphenol, an intermediate in the biosynthesis of ubiquinone-8 in Escherichia coli Saccharomyces cerevisiae S-adenosyl-L-homocysteine + 2-methoxy-6-(farnesyl)phenol i.e. 2-methoxy-6-((2E,6E)-3,7,11-trimethyldodeca-2,6,10-trienyl)phenol ?
2.1.1.222 S-adenosyl-L-methionine + 3-(all-trans-octaprenyl)benzene-1,2-diol
-
 Escherichia coli S-adenosyl-L-homocysteine + 2-methoxy-6-(all-trans-octaprenyl)phenol
-
 ?
2.1.1.222 S-adenosyl-L-methionine + 3-(all-trans-octaprenyl)benzene-1,2-diol the enzyme catalyzes two methylation steps in the biosynthesis of ubiquinone-8 in Escherichia coli, 1. the methylation of 3-(all-trans-octaprenyl)benzene-1,2-diol and 2. the methylation of 3-demethylubiquinol-8 (this reaction is classified as EC 2.1.1.64) Escherichia coli S-adenosyl-L-homocysteine + 2-methoxy-6-(all-trans-octaprenyl)phenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.64 COQ3
-
 Saccharomyces cerevisiae
2.1.1.64 COQ3
-
 Rattus norvegicus
2.1.1.64 UbiG
-
 Escherichia coli
2.1.1.114 Coq3p
-
 Saccharomyces cerevisiae
2.1.1.114 Coq3p
-
 Rattus norvegicus
2.1.1.222 UbiG
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.64 7
-
 assay at Escherichia coli
2.1.1.64 7
-
 assay at Saccharomyces cerevisiae
2.1.1.64 7
-
 assay at Rattus norvegicus
2.1.1.114 7
-
 assay at Saccharomyces cerevisiae
2.1.1.114 7
-
 assay at Rattus norvegicus
2.1.1.222 7
-
 assay at Escherichia coli
2.1.1.222 7
-
 assay at Saccharomyces cerevisiae
2.1.1.222 7
-
 assay at Rattus norvegicus

General Information

EC Number General Information Comment Organism
2.1.1.64 malfunction rat COQ3 gene restores O-methyltransferase activity in coq3 null mutant yeast Saccharomyces cerevisiae