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Literature summary extracted from

  • Bhatnagar, D.; Ullah, A.H.J.; Cleveland, T.E.
    Purification and characterization of a methyltransferase from Aspergillus parasiticus SRRC 163 involved in aflatoxin biosynthetic pathway (1988), Prep. Biochem., 18, 321-349.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.110 0.0018
-
 sterigmatocystin
-
 Aspergillus parasiticus
2.1.1.110 0.042
-
 S-adenosyl-L-methionine
-
 Aspergillus parasiticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.110 58000
-
 1 * 110000 + 1 * 58000, SRCC 163, SDS-PAGE Aspergillus parasiticus
2.1.1.110 110000
-
 1 * 110000 + 1 * 58000, SRCC 163, SDS-PAGE Aspergillus parasiticus
2.1.1.110 160000
-
 gel filtration Aspergillus parasiticus
2.1.1.110 168000
-
-
 Aspergillus parasiticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.110 S-adenosyl-L-methionine + sterigmatocystin Aspergillus parasiticus involved in the biosynthesis of aflatoxins in fungi S-adenosyl-L-homocysteine + 8-O-methylsterigmatocystin
-
 ?
2.1.1.110 S-adenosyl-L-methionine + sterigmatocystin Aspergillus parasiticus SRCC 163 involved in the biosynthesis of aflatoxins in fungi S-adenosyl-L-homocysteine + 8-O-methylsterigmatocystin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.110 Aspergillus parasiticus
-
 SRCC 163
-
2.1.1.110 Aspergillus parasiticus SRCC 163
-
 SRCC 163
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.110 NIAH-26 Aspergillus parasiticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.110 S-adenosyl-L-methionine + sterigmatocystin
-
 Aspergillus parasiticus S-adenosyl-L-homocysteine + 8-O-methylsterigmatocystin
-
 ?
2.1.1.110 S-adenosyl-L-methionine + sterigmatocystin involved in the biosynthesis of aflatoxins in fungi Aspergillus parasiticus S-adenosyl-L-homocysteine + 8-O-methylsterigmatocystin
-
 ?
2.1.1.110 S-adenosyl-L-methionine + sterigmatocystin
-
 Aspergillus parasiticus SRCC 163 S-adenosyl-L-homocysteine + 8-O-methylsterigmatocystin
-
 ?
2.1.1.110 S-adenosyl-L-methionine + sterigmatocystin involved in the biosynthesis of aflatoxins in fungi Aspergillus parasiticus SRCC 163 S-adenosyl-L-homocysteine + 8-O-methylsterigmatocystin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.110 dimer 1 * 110000 + 1 * 58000, SRCC 163, SDS-PAGE Aspergillus parasiticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.110 25 35
-
 Aspergillus parasiticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.110 0.022
-
 sterigmatocystin
-
 Aspergillus parasiticus
2.1.1.110 2.2
-
 sterigmatocystin
-
 Aspergillus parasiticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.110 7.5
-
-
 Aspergillus parasiticus
2.1.1.110 7.5 8
-
 Aspergillus parasiticus