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Literature summary extracted from

  • Yabe, K.; Matsushima, K.I.; Koyama, T.; Hamasaki, T.
    Purification and characterization of O-methyltransferase I involved in conversion of demethylsterigmatocystin to sterigmatocystin and of dihydrodemethylsterigmatocystin to dihydrosterigmatocystin during aflatoxin biosynthesis (1998), Appl. Environ. Microbiol., 64, 166-171.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.109 6-demethylsterigmatocystin at higher concentrations Aspergillus parasiticus
2.1.1.109 dihydrodemethylsterigmatocystin at higher concentrations Aspergillus parasiticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.109 0.00094
-
 6-demethylsterigmatocystin
-
 Aspergillus parasiticus
2.1.1.109 0.0025
-
 dihydrodemethylsterigmatocystin
-
 Aspergillus parasiticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.109 15000
-
 gel filtration Aspergillus parasiticus
2.1.1.109 43000
-
 x * 43000, SDS-PAGE Aspergillus parasiticus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.109 Aspergillus parasiticus
-
 NIAH-26
-
2.1.1.109 Aspergillus parasiticus NIAH-26
-
 NIAH-26
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.109 phenyl-Sepharose, Sephacryl S-300, Matrex gel Green A Aspergillus parasiticus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.109 0.043
-
 with dihydrodemethylsterigmatocystin Aspergillus parasiticus
2.1.1.109 0.061
-
 with 6-demethylsterigmatocystin Aspergillus parasiticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.109 S-adenosyl-L-methionine + 6-demethylsterigmatocystin
-
 Aspergillus parasiticus S-adenosyl-L-homocysteine + sterigmatocystin
-
 ?
2.1.1.109 S-adenosyl-L-methionine + 6-demethylsterigmatocystin
-
 Aspergillus parasiticus NIAH-26 S-adenosyl-L-homocysteine + sterigmatocystin
-
 ?
2.1.1.109 S-adenosyl-L-methionine + dihydrodemethylsterigmatocystin
-
 Aspergillus parasiticus S-adenosyl-L-homocysteine + dihydrosterigmatocystin
-
 ?
2.1.1.109 S-adenosyl-L-methionine + dihydrodemethylsterigmatocystin
-
 Aspergillus parasiticus NIAH-26 S-adenosyl-L-homocysteine + dihydrosterigmatocystin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.109 ? x * 43000, SDS-PAGE Aspergillus parasiticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.109 6.6 9
-
 Aspergillus parasiticus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.109 0.108
-
 6-demethylsterigmatocystin
-
 Aspergillus parasiticus
2.1.1.109 0.171
-
 dihydrodemethylsterigmatocystin
-
 Aspergillus parasiticus