EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.1.76 | MET8 mutants complemented by Salmonella typhimurium cysG, cloned and expressed in Escherichia coli | Saccharomyces cerevisiae |
2.1.1.107 | EC 2.1.1.107 deficient mutants complemented with cysG from Salmonella typhimurium and Escherichia coli and cobA from Pseudomonas denitrificans, overexpression of yeast gene product in Escherichia coli | Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.76 | precorrin-2 + NAD+ | Saccharomyces cerevisiae | siroheme and cobalamin biosynthesis, second of three steps leading to formation of siroheme from uroporphyrinogen III | sirohydrochlorin + NADH + H+ | - |
? | |
2.1.1.107 | 2 S-adenosyl-L-methionine + uroporphyrinogen III | Salmonella enterica subsp. enterica serovar Typhimurium | - |
2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? | |
2.1.1.107 | 2 S-adenosyl-L-methionine + uroporphyrinogen III | Escherichia coli | - |
2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? | |
2.1.1.107 | 2 S-adenosyl-L-methionine + uroporphyrinogen III | Pseudomonas denitrificans (nom. rej.) | - |
2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? | |
2.1.1.107 | 2 S-adenosyl-L-methionine + uroporphyrinogen III | Saccharomyces cerevisiae | involved in biosynthesis of siroheme | 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.76 | Saccharomyces cerevisiae | - |
yeast | - |
2.1.1.107 | Escherichia coli | - |
- |
- |
2.1.1.107 | Pseudomonas denitrificans (nom. rej.) | - |
- |
- |
2.1.1.107 | Saccharomyces cerevisiae | - |
- |
- |
2.1.1.107 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.1.76 | - |
Saccharomyces cerevisiae |
2.1.1.107 | from recombinant E. coli using His-tag | Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.76 | precorrin-2 + NAD+ | - |
Saccharomyces cerevisiae | sirohydrochlorin + NADH + H+ | - |
? | |
1.3.1.76 | precorrin-2 + NAD+ | siroheme and cobalamin biosynthesis, second of three steps leading to formation of siroheme from uroporphyrinogen III | Saccharomyces cerevisiae | sirohydrochlorin + NADH + H+ | - |
? | |
2.1.1.107 | 2 S-adenosyl-L-methionine + uroporphyrinogen III | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? | |
2.1.1.107 | 2 S-adenosyl-L-methionine + uroporphyrinogen III | - |
Escherichia coli | 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? | |
2.1.1.107 | 2 S-adenosyl-L-methionine + uroporphyrinogen III | - |
Saccharomyces cerevisiae | 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? | |
2.1.1.107 | 2 S-adenosyl-L-methionine + uroporphyrinogen III | - |
Pseudomonas denitrificans (nom. rej.) | 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? | |
2.1.1.107 | 2 S-adenosyl-L-methionine + uroporphyrinogen III | methylates positions 2 and 7 | Escherichia coli | 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? | |
2.1.1.107 | 2 S-adenosyl-L-methionine + uroporphyrinogen III | involved in biosynthesis of siroheme | Saccharomyces cerevisiae | 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.76 | cobaltochelatase | - |
Saccharomyces cerevisiae |
1.3.1.76 | Met8p | - |
Saccharomyces cerevisiae |