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Literature summary extracted from

  • Sattler, I.; Roessner, C.A.; Stolowich, N.J.; Hardin, S.H.; Harris-Haller, L.W.; Yokubaitis, N.T.; Murooka, Y.; Hashimoto, Y.; Scott, A.I.
    Cloning, sequencing, and expression of the uroporphyrinogen III methyltransferease cobA gene of Propionibacterium freudenreichii (shermanii) (1995), J. Bacteriol., 177, 1564-1569.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.107 overexpression of cobA gene in Escherichia coli TB1 Propionibacterium freudenreichii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.107 soluble
-
 Propionibacterium freudenreichii
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.107 27000
-
 SDS-PAGE Propionibacterium freudenreichii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.107 2 S-adenosyl-L-methionine + uroporphyrinogen III Propionibacterium freudenreichii involved in biosynthesis of sirohydrochlorin 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?
2.1.1.107 2 S-adenosyl-L-methionine + uroporphyrinogen III Propionibacterium freudenreichii involved in biosynthesis of cobalamin 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.107 Propionibacterium freudenreichii Q51720
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.107 2 S-adenosyl-L-methionine + uroporphyrinogen III
-
 Propionibacterium freudenreichii 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ after prolonged incubation (15 h) also tri- and tetra-methylated compounds formed, third methyl group at position 12 ?
2.1.1.107 2 S-adenosyl-L-methionine + uroporphyrinogen III
-
 Propionibacterium freudenreichii 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?
2.1.1.107 2 S-adenosyl-L-methionine + uroporphyrinogen III involved in biosynthesis of sirohydrochlorin Propionibacterium freudenreichii 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?
2.1.1.107 2 S-adenosyl-L-methionine + uroporphyrinogen III involved in biosynthesis of cobalamin Propionibacterium freudenreichii 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?
2.1.1.107 S-adenosyl-L-methionine + uroporphyrinogen I methylates positions 2 and 7 Propionibacterium freudenreichii ?
-
 ?