BRENDA - Enzyme Database

Nucleotide sequence of a Pseudomonas denitrificans 5.4-kilobase DNA fragment containing five cob genes and identification of structural genes encoding S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase and cobyrinic acid a,c-diamide synthase

Crouzet, J.; Cauchois, L.; Blanche, F.; Debussche, L.; Thibaut, D.; Rouyez, M.C.; Rigault, S.; Mayaux, J.F.; Cameron, B.; J. Bacteriol. 172, 5968-5979 (1990)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.1.1.107
expression of 5 open reading frames, codon 1120-1959 (cobA) encodes EC 2.1.1.107 activity
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.9
identification of the structural gene
Pseudomonas denitrificans (nomen rejiciendum)
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.107
29200
-
calculated from DNA sequence
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.9
45000
-
2 * 45000, homodimer
Pseudomonas denitrificans (nomen rejiciendum)
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.107
2 S-adenosyl-L-methionine + uroporphyrinogen III
Pseudomonas denitrificans (nomen rejiciendum)
involved in biosynthesis of cobalamin
2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+
product is precorrin-2
Pseudomonas denitrificans (nomen rejiciendum)
?
2.1.1.107
2 S-adenosyl-L-methionine + uroporphyrinogen III
Pseudomonas denitrificans (nomen rejiciendum) SC510
involved in biosynthesis of cobalamin
2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+
product is precorrin-2
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
6.3.5.9
additional information
Pseudomonas denitrificans (nomen rejiciendum)
cobalamin biosynthetic pathway
?
-
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
additional information
Pseudomonas denitrificans (nomen rejiciendum) SC510
cobalamin biosynthetic pathway
?
-
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.1.1.107
Pseudomonas denitrificans (nomen rejiciendum)
P21632
-
-
6.3.5.9
Pseudomonas denitrificans (nomen rejiciendum)
P21632
-
-
6.3.5.9
Pseudomonas denitrificans (nomen rejiciendum) SC510
P21632
-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.3.5.9
-
Pseudomonas denitrificans (nomen rejiciendum)
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.107
2 S-adenosyl-L-methionine + uroporphyrinogen III
-
485086
Pseudomonas denitrificans (nomen rejiciendum)
2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+
product is precorrin-2
485086
Pseudomonas denitrificans (nomen rejiciendum)
?
2.1.1.107
2 S-adenosyl-L-methionine + uroporphyrinogen III
involved in biosynthesis of cobalamin
485086
Pseudomonas denitrificans (nomen rejiciendum)
2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+
product is precorrin-2
485086
Pseudomonas denitrificans (nomen rejiciendum)
?
2.1.1.107
2 S-adenosyl-L-methionine + uroporphyrinogen III
-
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+
product is precorrin-2
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
2.1.1.107
2 S-adenosyl-L-methionine + uroporphyrinogen III
involved in biosynthesis of cobalamin
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+
product is precorrin-2
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
6.3.5.9
ATP + cobyrinic acid + L-glutamine + H2O
-
485086
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
485086
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
ATP + cobyrinic acid + L-glutamine + H2O
-
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
6.3.5.9
ATP + hydrogenobyrinic acid + L-glutamine + H2O
amidation of carboxylic groups at positions a and c
485086
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
485086
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
ATP + hydrogenobyrinic acid + L-glutamine + H2O
amidation of carboxylic groups at positions a and c
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
6.3.5.9
additional information
cobalamin biosynthetic pathway
485086
Pseudomonas denitrificans (nomen rejiciendum)
?
-
485086
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
additional information
cobalamin biosynthetic pathway
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
-
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
Subunits
EC Number
Subunits
Commentary
Organism
6.3.5.9
dimer
2 * 45000, homodimer
Pseudomonas denitrificans (nomen rejiciendum)
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.1.1.107
expression of 5 open reading frames, codon 1120-1959 (cobA) encodes EC 2.1.1.107 activity
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.9
identification of the structural gene
Pseudomonas denitrificans (nomen rejiciendum)
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.1.1.107
29200
-
calculated from DNA sequence
Pseudomonas denitrificans (nomen rejiciendum)
6.3.5.9
45000
-
2 * 45000, homodimer
Pseudomonas denitrificans (nomen rejiciendum)
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.1.1.107
2 S-adenosyl-L-methionine + uroporphyrinogen III
Pseudomonas denitrificans (nomen rejiciendum)
involved in biosynthesis of cobalamin
2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+
product is precorrin-2
Pseudomonas denitrificans (nomen rejiciendum)
?
2.1.1.107
2 S-adenosyl-L-methionine + uroporphyrinogen III
Pseudomonas denitrificans (nomen rejiciendum) SC510
involved in biosynthesis of cobalamin
2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+
product is precorrin-2
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
6.3.5.9
additional information
Pseudomonas denitrificans (nomen rejiciendum)
cobalamin biosynthetic pathway
?
-
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
additional information
Pseudomonas denitrificans (nomen rejiciendum) SC510
cobalamin biosynthetic pathway
?
-
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.3.5.9
-
Pseudomonas denitrificans (nomen rejiciendum)
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.1.1.107
2 S-adenosyl-L-methionine + uroporphyrinogen III
-
485086
Pseudomonas denitrificans (nomen rejiciendum)
2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+
product is precorrin-2
485086
Pseudomonas denitrificans (nomen rejiciendum)
?
2.1.1.107
2 S-adenosyl-L-methionine + uroporphyrinogen III
involved in biosynthesis of cobalamin
485086
Pseudomonas denitrificans (nomen rejiciendum)
2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+
product is precorrin-2
485086
Pseudomonas denitrificans (nomen rejiciendum)
?
2.1.1.107
2 S-adenosyl-L-methionine + uroporphyrinogen III
-
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+
product is precorrin-2
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
2.1.1.107
2 S-adenosyl-L-methionine + uroporphyrinogen III
involved in biosynthesis of cobalamin
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+
product is precorrin-2
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
6.3.5.9
ATP + cobyrinic acid + L-glutamine + H2O
-
485086
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
485086
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
ATP + cobyrinic acid + L-glutamine + H2O
-
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate
-
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
6.3.5.9
ATP + hydrogenobyrinic acid + L-glutamine + H2O
amidation of carboxylic groups at positions a and c
485086
Pseudomonas denitrificans (nomen rejiciendum)
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
485086
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
ATP + hydrogenobyrinic acid + L-glutamine + H2O
amidation of carboxylic groups at positions a and c
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate
-
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
6.3.5.9
additional information
cobalamin biosynthetic pathway
485086
Pseudomonas denitrificans (nomen rejiciendum)
?
-
485086
Pseudomonas denitrificans (nomen rejiciendum)
?
6.3.5.9
additional information
cobalamin biosynthetic pathway
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
-
485086
Pseudomonas denitrificans (nomen rejiciendum) SC510
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.3.5.9
dimer
2 * 45000, homodimer
Pseudomonas denitrificans (nomen rejiciendum)