EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.107 | expression of 5 open reading frames, codon 1120-1959 (cobA) encodes EC 2.1.1.107 activity | Pseudomonas denitrificans (nom. rej.) |
6.3.5.9 | identification of the structural gene | Pseudomonas denitrificans (nom. rej.) |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.107 | 29200 | - |
calculated from DNA sequence | Pseudomonas denitrificans (nom. rej.) |
6.3.5.9 | 45000 | - |
2 * 45000, homodimer | Pseudomonas denitrificans (nom. rej.) |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.107 | 2 S-adenosyl-L-methionine + uroporphyrinogen III | Pseudomonas denitrificans (nom. rej.) | involved in biosynthesis of cobalamin | 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? | |
2.1.1.107 | 2 S-adenosyl-L-methionine + uroporphyrinogen III | Pseudomonas denitrificans (nom. rej.) SC510 | involved in biosynthesis of cobalamin | 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? | |
6.3.5.9 | additional information | Pseudomonas denitrificans (nom. rej.) | cobalamin biosynthetic pathway | ? | - |
? | |
6.3.5.9 | additional information | Pseudomonas denitrificans (nom. rej.) SC510 | cobalamin biosynthetic pathway | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.107 | Pseudomonas denitrificans (nom. rej.) | P21632 | - |
- |
6.3.5.9 | Pseudomonas denitrificans (nom. rej.) | P21632 | - |
- |
6.3.5.9 | Pseudomonas denitrificans (nom. rej.) SC510 | P21632 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.3.5.9 | - |
Pseudomonas denitrificans (nom. rej.) |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.107 | 2 S-adenosyl-L-methionine + uroporphyrinogen III | - |
Pseudomonas denitrificans (nom. rej.) | 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? | |
2.1.1.107 | 2 S-adenosyl-L-methionine + uroporphyrinogen III | involved in biosynthesis of cobalamin | Pseudomonas denitrificans (nom. rej.) | 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? | |
2.1.1.107 | 2 S-adenosyl-L-methionine + uroporphyrinogen III | - |
Pseudomonas denitrificans (nom. rej.) SC510 | 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? | |
2.1.1.107 | 2 S-adenosyl-L-methionine + uroporphyrinogen III | involved in biosynthesis of cobalamin | Pseudomonas denitrificans (nom. rej.) SC510 | 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ | product is precorrin-2 | ? | |
6.3.5.9 | ATP + cobyrinic acid + L-glutamine + H2O | - |
Pseudomonas denitrificans (nom. rej.) | ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate | - |
? | |
6.3.5.9 | ATP + cobyrinic acid + L-glutamine + H2O | - |
Pseudomonas denitrificans (nom. rej.) SC510 | ADP + phosphate + cobyrinic acid a,c-diamide + L-glutamate | - |
? | |
6.3.5.9 | ATP + hydrogenobyrinic acid + L-glutamine + H2O | amidation of carboxylic groups at positions a and c | Pseudomonas denitrificans (nom. rej.) | ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate | - |
? | |
6.3.5.9 | ATP + hydrogenobyrinic acid + L-glutamine + H2O | amidation of carboxylic groups at positions a and c | Pseudomonas denitrificans (nom. rej.) SC510 | ADP + phosphate + hydrogenobyrinic acid a,c-diamide + L-glutamate | - |
? | |
6.3.5.9 | additional information | cobalamin biosynthetic pathway | Pseudomonas denitrificans (nom. rej.) | ? | - |
? | |
6.3.5.9 | additional information | cobalamin biosynthetic pathway | Pseudomonas denitrificans (nom. rej.) SC510 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.3.5.9 | dimer | 2 * 45000, homodimer | Pseudomonas denitrificans (nom. rej.) |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.3.5.9 | CobB | - |
Pseudomonas denitrificans (nom. rej.) |
6.3.5.9 | cobyrinic acid a,c-diamide synthase | - |
Pseudomonas denitrificans (nom. rej.) |