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Literature summary extracted from

  • Warren, M.J.; Roessner, C.A.; Santander, P.J.; Scott, A.I.
    The Escherichia coli cysG gene encodes S-adenosylmethionine-dependent uroporphyrinogen III methylase (1990), Biochem. J., 265, 725-729.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.107 cysG containing plasmid transformed into Escherichia coli CBK103 Escherichia coli
2.1.1.107 overexpression of cysG gene Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.107 49930
-
 calculated from DNA-sequence Escherichia coli
2.1.1.107 52000
-
 SDS-PAGE Escherichia coli
2.1.1.107 66000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.107 2 S-adenosyl-L-methionine + uroporphyrinogen III Escherichia coli involved in biosynthesis of sirohydrochlorin 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.107 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.107
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.107 2 S-adenosyl-L-methionine + uroporphyrinogen III methylates positions 2 and 7 Escherichia coli 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?
2.1.1.107 2 S-adenosyl-L-methionine + uroporphyrinogen III involved in biosynthesis of sirohydrochlorin Escherichia coli 2 S-adenosyl-L-homocysteine + dihydrosirohydrochlorin + 2 H+ product is precorrin-2 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.107 monomer SDS-PAGE, gel filtration Escherichia coli