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Literature summary extracted from

  • Pakusch, A.E.; Kneusel, R.E.; Matern, U.
    S-Adenosyl-L-methionine:trans-caffeoyl-coenzyme A 3-O-methyltransferase from elicitor-treated parsley cell suspension cultures (1989), Arch. Biochem. Biophys., 271, 488-494.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.104 ethanol activation, 12% v/v Petroselinum crispum
2.1.1.104 Mg2+ activation, 0.2 mM Petroselinum crispum
2.1.1.104 NaCl activation, 0.4 M Petroselinum crispum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.104 Ca2+
-
 Petroselinum crispum
2.1.1.104 EDTA
-
 Petroselinum crispum
2.1.1.104 Mn2+
-
 Petroselinum crispum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.104 Mg2+ activation, 0.2 mM Petroselinum crispum
2.1.1.104 NaCl activation, 0.4 M Petroselinum crispum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.104 48000
-
 gel filtration Petroselinum crispum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.104 S-adenosyl-L-methionine + caffeoyl-CoA Petroselinum crispum inducible S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.104 Petroselinum crispum
-
 parsley
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.104
-
 Petroselinum crispum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.104 cell suspension culture treated with crude cell wall elicitor from Phytophthora megasperma f.sp. glycinea Petroselinum crispum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.104 0.059
-
-
 Petroselinum crispum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.104 additional information no substrates are caffeic acid, bergaptol, xanthotoxol, luteolin, esculetin Petroselinum crispum ?
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeoyl-CoA trans-caffeoyl-CoA preferred Petroselinum crispum S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeoyl-CoA inducible Petroselinum crispum S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?
2.1.1.104 S-adenosyl-L-methionine + trans-caffeic acid esters such as methyl caffeate, chlorogenic acid, trans-5-O-caffeoylshikimate, rosmarinic acid, poor substrates Petroselinum crispum S-adenosyl-L-homocysteine + ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.104 30
-
 assay at Petroselinum crispum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.104 7.5
-
-
 Petroselinum crispum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.104 6.9 8.6 about half-maximal activity at pH 6.9 and 8.6 Petroselinum crispum