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Literature summary extracted from

  • Grimmig, B.; Kneusel, R.E.; Junghanns, K.T.; Matern, U.
    Expression of bifunctional caffeoyl-CoA 3-O-methyltransferase in stress compensation and lignification (1999), Plant Biol., 1, 299-310.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.104 expression in Escherichia coli JM109 DE3 Petroselinum crispum
2.1.1.104 transformed with parsley gene Nicotiana tabacum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.104 0.00024
-
 caffeoyl-CoA
-
 Petroselinum crispum
2.1.1.104 0.0007
-
 5-hydroxyferuoyl-CoA
-
 Petroselinum crispum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.104 27000
-
-
 Nicotiana tabacum
2.1.1.104 27000
-
 gel filtration, recombinant protein Petroselinum crispum
2.1.1.104 30000
-
 gel filtration, crude extract Petroselinum crispum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.104 S-adenosyl-L-methionine + caffeoyl-CoA Petroselinum crispum bi-functional enzyme, involved in lignin biosynthesis S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeoyl-CoA Petroselinum crispum enzyme plays a pivotal role in cell wall reinforcement during the induced disease resistance response S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.68 Petroselinum crispum
-
 parsley
-
2.1.1.104 Nicotiana tabacum
-
 cv Havana SR1
-
2.1.1.104 Petroselinum crispum
-
 parsley
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.68 stem
-
 Petroselinum crispum
-
2.1.1.104 leaf
-
 Nicotiana tabacum
-
2.1.1.104 leaf
-
 Petroselinum crispum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.68 S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate
-
 Petroselinum crispum S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
-
 ?
2.1.1.68 S-adenosyl-L-methionine + 5-hydroxyferulic acid
-
 Petroselinum crispum S-adenosyl-L-homocysteine + sinapic acid
-
 ?
2.1.1.104 S-adenosyl-L-methionine + 5-hydroxyferuoyl-CoA
-
 Nicotiana tabacum S-adenosyl-L-homocysteine + sinapoyl-CoA
-
 ?
2.1.1.104 S-adenosyl-L-methionine + 5-hydroxyferuoyl-CoA
-
 Petroselinum crispum S-adenosyl-L-homocysteine + sinapoyl-CoA
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeoyl-CoA
-
 Nicotiana tabacum S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeoyl-CoA
-
 Petroselinum crispum S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeoyl-CoA bi-functional enzyme, involved in lignin biosynthesis Petroselinum crispum S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?
2.1.1.104 S-adenosyl-L-methionine + caffeoyl-CoA enzyme plays a pivotal role in cell wall reinforcement during the induced disease resistance response Petroselinum crispum S-adenosyl-L-homocysteine + feruloyl-CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.104 CCoAOMT
-
 Nicotiana tabacum