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Literature summary extracted from

  • Nuccio, M.L.; Ziemak, M.J.; Henry, S.A.; Weretilnyk, E.A.; Hanson, A.D.
    cDNA cloning of phosphoethanolamine N-methyltransferase from spinach by complementation in Schizosaccharomyces pombe and characterization of the recombinant enzyme (2000), J. Biol. Chem., 275, 14095-14101.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.103 in Schizosaccharomyces pombe Spinacia oleracea

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.103 additional information deleting the C-terminal domain does not abolish ethanolamine phosphate reactivity, C-terminal domain catalyzes reactions with methylated ethanolamine phosphate Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.103 phosphocholine
-
 Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.103 0.096
-
 Ethanolamine phosphate
-
 Spinacia oleracea
2.1.1.103 0.132
-
 S-adenosyl-L-methionine
-
 Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.103 57000
-
 gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate Spinacia oleracea involved in synthesis of phosphocholine and phosphatidylcholine S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate Spinacia oleracea involved in production of free choline S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.103 Spinacia oleracea Q9M571
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.103 leaf
-
 Spinacia oleracea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.103 S-adenosyl-L-methionine + 2-(methylamino)ethyl phosphate
-
 Spinacia oleracea S-adenosyl-L-homocysteine + 2-(dimethylamino)ethyl phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate
-
 Spinacia oleracea S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate involved in synthesis of phosphocholine and phosphatidylcholine Spinacia oleracea S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + ethanolamine phosphate involved in production of free choline Spinacia oleracea S-adenosyl-L-homocysteine + N-methylethanolamine phosphate
-
 ?
2.1.1.103 S-adenosyl-L-methionine + phosphodimethylethanolamine
-
 Spinacia oleracea S-adenosyl-L-homocysteine + phosphocholine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.103 monomer gel filtration Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.103 7.8 8.5
-
 Spinacia oleracea