EC Number | Application | Comment | Organism |
---|---|---|---|
2.1.1.101 | pharmacology | tylosin fermentation, antibiotic biosynthesis, enzyme catalyzes conversion of macrocin to tylosin in vivo | Streptomyces fradiae |
2.1.1.102 | pharmacology | tylosin fermentation, antibiotic biosynthesis | Streptomyces fradiae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.101 | A9145C | - |
Streptomyces fradiae | |
2.1.1.101 | demethyllactenocin | - |
Streptomyces fradiae | |
2.1.1.101 | Demethylmacrocin | - |
Streptomyces fradiae | |
2.1.1.101 | Desmycosin | weak inhibition | Streptomyces fradiae | |
2.1.1.101 | Relomycin | weak inhibition | Streptomyces fradiae | |
2.1.1.101 | S-adenosyl-L-homocysteine | not D-enantiomer | Streptomyces fradiae | |
2.1.1.101 | sinefungin | competitive inhibition, potent inhibitor | Streptomyces fradiae | |
2.1.1.101 | tylosin | weak inhibition | Streptomyces fradiae | |
2.1.1.102 | 20-Dihydro-23-deoxymycaminosyltylonolide | - |
Streptomyces fradiae | |
2.1.1.102 | 20-dihydrolactenocin | inhibition of macrocin formation | Streptomyces fradiae | |
2.1.1.102 | 20-Dihydromacrocin | - |
Streptomyces fradiae | |
2.1.1.102 | 23-demycinosyloxytylosin | - |
Streptomyces fradiae | |
2.1.1.102 | 23-Deoxy-O-mycaminosyltylonolide | - |
Streptomyces fradiae | |
2.1.1.102 | A9145C | - |
Streptomyces fradiae | |
2.1.1.102 | demycinosyltylosin | - |
Streptomyces fradiae | |
2.1.1.102 | Fe2+ | - |
Streptomyces fradiae | |
2.1.1.102 | lactenocin | inhibition of macrocin formation | Streptomyces fradiae | |
2.1.1.102 | macrocin | inhibition of lactenocin formation | Streptomyces fradiae | |
2.1.1.102 | mycaminosyltylactone | - |
Streptomyces fradiae | |
2.1.1.102 | mycaminosyltylonolide | - |
Streptomyces fradiae | |
2.1.1.102 | O-Mycaminosyltylonolide | - |
Streptomyces fradiae | |
2.1.1.102 | S-adenosyl-L-homocysteine | not D-enantiomer | Streptomyces fradiae | |
2.1.1.102 | sinefungin | competitive inhibition, poor inhibitor | Streptomyces fradiae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.101 | 0.005 | - |
macrocin | - |
Streptomyces fradiae | |
2.1.1.101 | 0.007 | - |
lactenocin | - |
Streptomyces fradiae | |
2.1.1.101 | 0.022 | - |
S-adenosyl-L-methionine | lactenocin as substrate | Streptomyces fradiae | |
2.1.1.101 | 0.023 | - |
S-adenosyl-L-methionine | macrocin as substrate | Streptomyces fradiae | |
2.1.1.102 | 0.006 | - |
Demethylmacrocin | - |
Streptomyces fradiae | |
2.1.1.102 | 0.01 | - |
demethyllactenocin | - |
Streptomyces fradiae | |
2.1.1.102 | 0.111 | - |
S-adenosylmethionine | - |
Streptomyces fradiae |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.101 | Co2+ | can substitute Mg2+ | Streptomyces fradiae | |
2.1.1.101 | Mg2+ | required for maximal activity | Streptomyces fradiae | |
2.1.1.101 | Mn2+ | can substitute Mg2+ | Streptomyces fradiae | |
2.1.1.102 | Mg2+ | required for maximal activity | Streptomyces fradiae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.101 | 32000 | - |
2 * 32000, SDS-PAGE | Streptomyces fradiae |
2.1.1.101 | 65000 | - |
- |
Streptomyces fradiae |
2.1.1.102 | 42000 | - |
3 * 42000, SDS-PAGE | Streptomyces fradiae |
2.1.1.102 | 122000 | 126000 | gel filtration | Streptomyces fradiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.101 | S-adenosyl-L-methionine + macrocin | Streptomyces fradiae | enzyme catalyzes 3'''-O-methylation of bound 2'''-O-methylated 6-deoxy-D-allose | S-adenosylhomocysteine + tylosin | - |
? | |
2.1.1.102 | S-adenosyl-L-methionine + demethylmacrocin | Streptomyces fradiae | enzyme catalyzes 2'''-O-methylation of bound 6-deoxy-D-allose | S-adenosyl-L-homocysteine + macrocin | - |
? | |
2.1.1.102 | S-adenosyl-L-methionine + demethylmacrocin | Streptomyces fradiae | penultimate step in tylosin biosynthesis | S-adenosyl-L-homocysteine + macrocin | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.101 | Streptomyces fradiae | - |
- |
- |
2.1.1.102 | Streptomyces fradiae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.101 | - |
Streptomyces fradiae |
2.1.1.102 | - |
Streptomyces fradiae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.1.102 | 0.25 | - |
- |
Streptomyces fradiae |
EC Number | Storage Stability | Organism |
---|---|---|
2.1.1.101 | 4°C, quite stable in presence of 0.2 mM S-adenosyl-L-methionine and 10% ethanol, pH 7.0, stable over 6 months | Streptomyces fradiae |
2.1.1.102 | 4°C, quite stable in presence of S-adenosylmethionine and 10% ethanol, pH 7.0-8.0, stable for a minimum of 2 months | Streptomyces fradiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.101 | S-adenosyl-L-methionine + lactenocin | - |
Streptomyces fradiae | S-adenosyl-L-homocysteine + desmycosin | - |
? | |
2.1.1.101 | S-adenosyl-L-methionine + macrocin | - |
Streptomyces fradiae | S-adenosyl-L-homocysteine + tylosin | - |
? | |
2.1.1.101 | S-adenosyl-L-methionine + macrocin | enzyme catalyzes 3'''-O-methylation of bound 2'''-O-methylated 6-deoxy-D-allose | Streptomyces fradiae | S-adenosylhomocysteine + tylosin | - |
? | |
2.1.1.102 | S-adenosyl-L-methionine + demethyllactenocin | - |
Streptomyces fradiae | S-adenosyl-L-homocysteine + lactenocin | - |
? | |
2.1.1.102 | S-adenosyl-L-methionine + demethylmacrocin | - |
Streptomyces fradiae | S-adenosyl-L-homocysteine + macrocin | - |
? | |
2.1.1.102 | S-adenosyl-L-methionine + demethylmacrocin | enzyme catalyzes 2'''-O-methylation of bound 6-deoxy-D-allose | Streptomyces fradiae | S-adenosyl-L-homocysteine + macrocin | - |
? | |
2.1.1.102 | S-adenosyl-L-methionine + demethylmacrocin | penultimate step in tylosin biosynthesis | Streptomyces fradiae | S-adenosyl-L-homocysteine + macrocin | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.101 | dimer | 2 * 32000, SDS-PAGE | Streptomyces fradiae |
2.1.1.102 | trimer | 3 * 42000, SDS-PAGE | Streptomyces fradiae |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.101 | 31 | - |
- |
Streptomyces fradiae |
2.1.1.102 | 42 | - |
- |
Streptomyces fradiae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.101 | 7.8 | 8 | - |
Streptomyces fradiae |
2.1.1.102 | 7.8 | 8.5 | - |
Streptomyces fradiae |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.101 | 0.00006 | - |
sinefungin | - |
Streptomyces fradiae | |
2.1.1.102 | 0.022 | - |
lactenocin | fixed substrate demethylmacrocin | Streptomyces fradiae | |
2.1.1.102 | 0.024 | - |
lactenocin | fixed substrate S-adenosylmethionine | Streptomyces fradiae | |
2.1.1.102 | 0.037 | - |
macrocin | fixed substrate S-adenosylmethionine | Streptomyces fradiae | |
2.1.1.102 | 0.057 | - |
A9145C | fixed substrate demethylmacrocin | Streptomyces fradiae | |
2.1.1.102 | 0.065 | - |
mycaminosyltylonolide | - |
Streptomyces fradiae | |
2.1.1.102 | 0.067 | - |
macrocin | fixed substrate demethyllactenocin | Streptomyces fradiae | |
2.1.1.102 | 0.073 | - |
23-Deoxy-O-mycaminosyltylonolide | - |
Streptomyces fradiae | |
2.1.1.102 | 0.077 | - |
sinefungin | - |
Streptomyces fradiae | |
2.1.1.102 | 0.108 | - |
A9145C | fixed substrate S-adenosylmethionine | Streptomyces fradiae | |
2.1.1.102 | 0.11 | - |
S-adenosyl-L-homocysteine | fixed substrate demethylmacrocin | Streptomyces fradiae | |
2.1.1.102 | 0.186 | - |
23-demycinosyloxytylosin | - |
Streptomyces fradiae | |
2.1.1.102 | 0.226 | - |
S-adenosyl-L-homocysteine | fixed substrate S-adenosylmethionine | Streptomyces fradiae |