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Literature summary extracted from

  • Bauer, N.J.; Kreuzman, A.J.; Dotzlaf, J.E.; Yeh, W.K.
    Purification, characterization, and kinetic mechanism of S-adenosyl-L-methionine:macrocin O-methyltransferase from Streptomyces fradiae (1988), J. Biol. Chem., 263, 15619-15625.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.101 agriculture tylosin is used in the swine industry as a growth promotant Streptomyces fradiae
2.1.1.101 pharmacology tylosin is used in veterinary medicine for treatment of infections caused by gram-positive bacteria and mycoplasma Streptomyces fradiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.101 demethyllactenocin
-
 Streptomyces fradiae
2.1.1.101 Demethylmacrocin
-
 Streptomyces fradiae
2.1.1.101 Desmycosin weak inhibition Streptomyces fradiae
2.1.1.101 Relomycin weak inhibition Streptomyces fradiae
2.1.1.101 S-adenosyl-L-homocysteine
-
 Streptomyces fradiae
2.1.1.101 sinefungin competitive inhibition, potent inhibitor Streptomyces fradiae
2.1.1.101 tylosin
-
 Streptomyces fradiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.101 Co2+ can substitute Mg2+ Streptomyces fradiae
2.1.1.101 Mg2+ required for maximal activity Streptomyces fradiae
2.1.1.101 Mn2+ can substitute Mg2+ Streptomyces fradiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.101 32000
-
 2 * 32000, SDS-PAGE Streptomyces fradiae
2.1.1.101 65000
-
 gel filtration Streptomyces fradiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.101 S-adenosyl-L-methionine + macrocin Streptomyces fradiae terminal and main rate-limiting step in tylosin biosynthesis S-adenosylhomocysteine + tylosin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.101 Streptomyces fradiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.101
-
 Streptomyces fradiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.101 0.061
-
-
 Streptomyces fradiae

Storage Stability

EC Number Storage Stability Organism
2.1.1.101 4°C, quite stable in presence of 0.2 mM S-adenosyl-L-methionine and 10% ethanol, pH 7.0, stable over 6 months Streptomyces fradiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.101 additional information demethylmacrocin, demethyllactenocin, N5-methyltetrahydrofolate, betaine, choline, methylthioadenosine and methionine are not substrates Streptomyces fradiae ?
-
 ?
2.1.1.101 S-adenosyl-L-methionine + 20-dihydrolactenocin
-
 Streptomyces fradiae S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.101 S-adenosyl-L-methionine + dihydromacrocin
-
 Streptomyces fradiae S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.101 S-adenosyl-L-methionine + lactenocin
-
 Streptomyces fradiae S-adenosyl-L-homocysteine + desmycosin
-
 ?
2.1.1.101 S-adenosyl-L-methionine + macrocin
-
 Streptomyces fradiae S-adenosyl-L-homocysteine + tylosin
-
 ?
2.1.1.101 S-adenosyl-L-methionine + macrocin terminal and main rate-limiting step in tylosin biosynthesis Streptomyces fradiae S-adenosylhomocysteine + tylosin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.101 dimer 2 * 32000, SDS-PAGE Streptomyces fradiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.101 31
-
-
 Streptomyces fradiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.101 7.5 8
-
 Streptomyces fradiae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.101 0.00006
-
 sinefungin
-
 Streptomyces fradiae
2.1.1.101 0.00025
-
 sinefungin
-
 Streptomyces fradiae
2.1.1.101 0.005
-
 Demethylmacrocin
-
 Streptomyces fradiae
2.1.1.101 0.006
-
 4'-demethyllactenocin
-
 Streptomyces fradiae
2.1.1.101 0.008
-
 S-adenosyl-L-homocysteine fixed substrate macrocin Streptomyces fradiae
2.1.1.101 0.023
-
 Desmycosin fixed substrate S-adenosyl-L-methionine Streptomyces fradiae
2.1.1.101 0.025
-
 S-adenosyl-L-homocysteine fixed substrate S-adenosyl-L-methionine Streptomyces fradiae
2.1.1.101 0.026
-
 Desmycosin fixed substrate macrocin Streptomyces fradiae
2.1.1.101 0.079
-
 tylosin fixed substrate S-adenosyl-L-methionine Streptomyces fradiae
2.1.1.101 0.217
-
 tylosin fixed substrate lactenocin Streptomyces fradiae