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Literature summary extracted from

  • Su, Y.; Varughese, K.I.; Xuong, N.H.; Bray, T.L.; Roche, D.J.; Whiteley, J.M.
    The crystallographic structure of a human dihydropteridine reductase NADH binary complex expressed in Escherichia coli by a cDNA constructed from its rat homologue (1993), J. Biol. Chem., 268, 26836-26841.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.1.34 hanging drop vapour diffusion, polyethylene glycol 4500, various concentrations of Tris phosphate buffer, pH 7.8, 8% methanol and 1 mM beta-mercaptoethanol, 2.5 A resolution Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.34 0.00015
-
 quinonoid 7,8-dihydrobiopterin recombinant enzyme Homo sapiens
1.5.1.34 0.009
-
 NADH recombinant enzyme Homo sapiens
1.5.1.34 0.018
-
 quinonoid 2-amino-4-hydroxy-6,7-dimethyl-7,8-dihydropteridine recombinant enzyme Homo sapiens
1.5.1.34 0.135
-
 NADPH recombinant enzyme Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.34 Homo sapiens
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.34 412
-
 recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.34 6,7-dihydropteridine + NADH
-
 Homo sapiens (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + NAD+
-
 ?
1.5.1.34 6,7-dimethyl-7,8-dihydropterin + NADPH + H+
-
 Homo sapiens 6,7-dimethyl-5,6,7,8-tetrahydropterin + NADP+
-
 ?
1.5.1.34 quinoid dihydrobiopterin + NAD(P)H
-
 Homo sapiens tetrahydrobiopterin + NAD(P)+
-
 ?
1.5.1.34 quinonoid dihydrobiopterin + NADH + H+
-
 Homo sapiens 6R-(1'R,2'R)-5,6,7,8-tetrahydrobiopterin + NAD+
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.34 17
-
 NADH recombinant enzyme Homo sapiens
1.5.1.34 17
-
 quinoid 7,8-dihydrobiopterin recombinant enzyme Homo sapiens
1.5.1.34 20
-
 NADPH recombinant enzyme Homo sapiens
1.5.1.34 162
-
 quinoid 2-amino-4-hydroxy-6,7-dimethyl-7,8-dihydropteridine recombinant enzyme Homo sapiens