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Literature summary extracted from

  • Nakanishi, N.; Hasegawa, H.; Akino, M.; Yamada, S.
    Catalytic properties of NADPH-specific dihydropteridine reductase from bovine liver (1986), J. Biochem., 99, 645-652.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.34 2,6-dichlorophenolindophenol inhibition of NADPH-specific enzyme Bos taurus
1.5.1.34 aminopterin 0.025 mM, 50% inhibition of NADH-specific enzyme, 1 mM, 50% inhibition of NADPH-specific enzyme Bos taurus
1.5.1.34 L-thyroxine mixed-type inhibition Bos taurus
1.5.1.34 methopterin 0.038 mM, 50% inhibition of NADH-specific enzyme; 0.7 mM, 50% inhibition of NADPH-specific enzyme Bos taurus
1.5.1.34 NADP+ competitive vs. NADPH Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.34 0.0011
-
 tetrahydrobiopterin NADH-specific liver enzyme, cofactor NADPH Bos taurus
1.5.1.34 0.0014
-
 6-methyl-tetrahydropterin NADPH-specific liver enzyme, cofactor NADPH Bos taurus
1.5.1.34 0.0014
-
 NADPH NADPH specific liver enzyme, substrate 6-methyl-tetrahydropterin Bos taurus
1.5.1.34 0.0015
-
 NADH NADH-specific liver enzyme, substrate tetrahydrobiopterin Bos taurus
1.5.1.34 0.0016
-
 tetrahydrobiopterin NADH-specific liver enzyme, cofactor NADH Bos taurus
1.5.1.34 0.0017
-
 NADPH NADPH specific liver enzyme, substrate tetrahydrobiopterin Bos taurus
1.5.1.34 0.0056
-
 6-methyl-tetrahydropterin NADH-specific liver enzyme, cofactor NADH Bos taurus
1.5.1.34 0.0058
-
 6-methyl-tetrahydropterin NADH-specific liver enzyme, cofactor NADPH Bos taurus
1.5.1.34 0.0068
-
 tetrahydrobiopterin NADPH-specific liver enzyme, cofactor NADPH Bos taurus
1.5.1.34 0.013
-
 6-methyl-tetrahydropterin NADPH-specific liver enzyme, cofactor NADH Bos taurus
1.5.1.34 0.074
-
 NADH NADH specific liver enzyme, substrate tetrahydrobiopterin Bos taurus
1.5.1.34 0.095
-
 NADPH NADH specific liver enzyme, substrate 6-methyl-tetrahydropterin Bos taurus
1.5.1.34 2.9
-
 NADH NADPH specific liver enzyme, substrate 6-methyltetrahydropterin Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.34 Bos taurus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.34 liver
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.34 quinonoid 6-methyl-7,8-(6H)-dihydropterin + NAD(P)H
-
 Bos taurus 6-methyl-5,6,7,8-tetrahydropterin + NAD(P)+
-
 ?
1.5.1.34 quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H
-
 Bos taurus 5,6,7,8-tetrahydrobiopterin + NAD(P)+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.34 NADH liver: 2 distinct dihydropteridine reductases which catalyze the conversion of the quinonoid dihydropteridine to tetrahydropterin: NADH-dihydropteridine reductase, DPR, utilizes NADH as a better substrate than NADPH, NADPH-specific dihydropteridine reductase, TPR, shows strict specificity for NADPH Bos taurus
1.5.1.34 NADPH liver: 2 distinct dihydropteridine reductases which catalyze the conversion of the quinonoid dihydropteridine to tetrahydropterin: NADH-dihydropteridine reductase, DPR, utilizes NADH as a better substrate than NADPH, NADPH-specific dihydropteridine reductase, TPR, shows strict specificity for NADPH Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.34 0.0032
-
 NADP+
-
 Bos taurus
1.5.1.34 0.016
-
 L-thyroxine
-
 Bos taurus