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Literature summary extracted from

  • Vasudevan, S.G.; Shaw, D.C.; Armarego, W.L.F.
    Dihydropteridine reductase from Escherichia coli (1988), Biochem. J., 255, 581-588.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.34 methotrexate competitive vs. quinoid 6-methyl-7,8-dihydropterin Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.34 0.091
-
 NADH
-
 Escherichia coli
1.5.1.34 0.128
-
 quinonoid-6-methyl-7,8-dihydrobiopterin
-
 Escherichia coli
1.5.1.34 0.226
-
 quinonoid dihydrobiopterin
-
 Escherichia coli
1.5.1.34 0.29
-
 NADPH
-
 Escherichia coli
1.5.1.34 0.525
-
 quinonoid-6,7-dimethyl-7,8-dihydropterin
-
 Escherichia coli
1.5.1.34 1.05
-
 Quinonoid dihydroneopterin
-
 Escherichia coli
1.5.1.34 1.32
-
 Quinonoid dihydromonapterin
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.34 27000
-
 2 * 27000, SDS-PAGE Escherichia coli
1.5.1.34 27000
-
 2 * 27000 Escherichia coli
1.5.1.34 54000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.34 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.34
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.34 20.7
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.34 6,7-dimethyl-7,8-dihydropteridine + NADPH
-
 Escherichia coli (6R)-L-erythro-6,7-dimethyl-5,6,7,8-tetrahydrobiopterin + NADP+
-
 ?
1.5.1.34 K3Fe(CN)6 + NAD(P)H
-
 Escherichia coli K4Fe(CN)6 + NAD(P)+
-
 ?
1.5.1.34 quinonoid 6,7-dimethyl-7,8-(6H)-dihydropterin + NAD(P)H
-
 Escherichia coli 6,7-dimethyl-5,6,7,8-tetrahydropterin + NAD(P)+
-
 ?
1.5.1.34 quinonoid 6-methyl-7,8-(6H)-dihydropterin + NAD(P)H
-
 Escherichia coli 6-methyl-5,6,7,8-tetrahydropterin + NAD(P)+
-
 ?
1.5.1.34 quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H enzyme also has pterin-independent NADH and NADPH oxidoreductase activity with potassium ferricyanide Escherichia coli 5,6,7,8-tetrahydrobiopterin + NAD(P)+
-
 ?
1.5.1.34 quinonoid dihydrobiopterin + NADH + H+
-
 Escherichia coli 6R-(1'R,2'R)-5,6,7,8-tetrahydrobiopterin + NAD+
-
 ?
1.5.1.34 quinonoid dihydromonapterin + NAD(P)H
-
 Escherichia coli tetrahydromonapterin + NAD(P)+
-
 ?
1.5.1.34 quinonoid dihydroneopterin + NAD(P)H
-
 Escherichia coli tetrahydroneopterin + NAD(P)+
-
 ?
1.5.1.34 quinonoid-6-methyl-7,8-dihydrobiopterin + NADH + H+
-
 Escherichia coli 6-methyl-7,8-dihydropterin + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.5.1.34 dimer 2 * 27000, SDS-PAGE Escherichia coli
1.5.1.34 dimer 2 * 27000 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.34 FAD contains bound FAD Escherichia coli
1.5.1.34 NADH utilizes both NADH and NADPH Escherichia coli
1.5.1.34 NADPH utilizes both NADH and NADPH Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.34 0.26
-
 methotrexate
-
 Escherichia coli