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Literature summary extracted from

  • Craine, J.E.; Hall, E.S.; Kaufman, S.
    The isolation and characterization of dihydropteridine reductase from sheep liver (1972), J. Biol. Chem., 247, 6082-6091.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.34 methotrexate
-
 Ovis aries

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.34 0.0009
-
 tetrahydrobiopterin cofactor NADPH Ovis aries
1.5.1.34 0.001
-
 tetrahydrobiopterin cofactor NADH Ovis aries
1.5.1.34 0.0011
-
 tetrahydrobiopterin
-
 Homo sapiens
1.5.1.34 0.0011
-
 tetrahydrobiopterin
-
 Rattus norvegicus
1.5.1.34 0.0022
-
 NADH
-
 Rattus norvegicus
1.5.1.34 0.004
-
 NADH substrate tetrahydrobiopterin Ovis aries
1.5.1.34 0.0057
-
 NADH substrate 6,7-dimethyl-5,6,7,8-tetrahydrobiopterin Ovis aries
1.5.1.34 0.0063
-
 6,7-dimethyl-5,6,7,8-tetrahydrobiopterin cofactor NADPH Ovis aries
1.5.1.34 0.008
-
 NADH
-
 Homo sapiens
1.5.1.34 0.0152
-
 6,7-dimethyl-5,6,7,8-tetrahydrobiopterin cofactor NADH Ovis aries
1.5.1.34 0.0709
-
 NADPH substrate tetrahydrobiopterin Ovis aries
1.5.1.34 0.0806
-
 NADPH substrate 6,7-dimethyl-5,6,7,8-tetrahydrobiopterin Ovis aries
1.5.1.34 0.1
-
 NADPH
-
 Rattus norvegicus
1.5.1.34 0.11
-
 NADPH
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.34 21000
-
 2 * 21000, liver, SDS-PAGE Ovis aries
1.5.1.34 41000
-
 gradient PAGE Ovis aries

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.34 Homo sapiens
-
-
-
1.5.1.34 Ovis aries
-
-
-
1.5.1.34 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.34
-
 Homo sapiens
1.5.1.34
-
 Rattus norvegicus
1.5.1.34
-
 Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.34 adrenal medulla
-
 Ovis aries
-
1.5.1.34 brain
-
 Homo sapiens
-
1.5.1.34 brain
-
 Ovis aries
-
1.5.1.34 kidney
-
 Ovis aries
-
1.5.1.34 liver
-
 Homo sapiens
-
1.5.1.34 liver
-
 Rattus norvegicus
-
1.5.1.34 liver
-
 Ovis aries
-
1.5.1.34 spleen
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.34 2.51
-
-
 Homo sapiens
1.5.1.34 3.64
-
-
 Rattus norvegicus
1.5.1.34 15.6
-
-
 Ovis aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.34 6,7-dihydropteridine + NADH
-
 Homo sapiens (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + NAD+
-
 ?
1.5.1.34 6,7-dimethyl-5,6,7,8-tetrahydropterin + NAD+
-
 Ovis aries 6,7-dimethyl-7,8-dihydropterin + NADH + H+
-
 r
1.5.1.34 6,7-dimethyl-5,6,7,8-tetrahydropterin + NADP+
-
 Ovis aries 6,7-dimethyl-7,8-dihydropterin + NADPH + H+
-
 r
1.5.1.34 quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H
-
 Ovis aries 5,6,7,8-tetrahydrobiopterin + NAD(P)+
-
 ?
1.5.1.34 quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H enzyme prefers NADH Ovis aries 5,6,7,8-tetrahydrobiopterin + NAD(P)+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.34 dimer
-
 Homo sapiens
1.5.1.34 dimer
-
 Rattus norvegicus
1.5.1.34 dimer 2 * 21000, liver, SDS-PAGE Ovis aries

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.34 NADH utilizes both NADH and NADPH Homo sapiens
1.5.1.34 NADH utilizes both NADH and NADPH Rattus norvegicus
1.5.1.34 NADH utilizes both NADH and NADPH Ovis aries
1.5.1.34 NADH enzyme prefers NADH Homo sapiens
1.5.1.34 NADH enzyme prefers NADH Rattus norvegicus
1.5.1.34 NADH enzyme prefers NADH Ovis aries
1.5.1.34 NADPH utilizes both NADH and NADPH Homo sapiens
1.5.1.34 NADPH utilizes both NADH and NADPH Rattus norvegicus
1.5.1.34 NADPH utilizes both NADH and NADPH Ovis aries
1.5.1.34 NADPH NADH is preferred Homo sapiens
1.5.1.34 NADPH NADH is preferred Rattus norvegicus
1.5.1.34 NADPH NADH is preferred Ovis aries

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.34 0.038
-
 methotrexate
-
 Ovis aries