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Literature summary extracted from

  • Williams, C.D.; Dickens, G.; Letendre, C.H.; Guroff, G.; Haines, C.; Shiota, T.
    Isolation and characterization of dihydropteridine reductase from Pseudomonas species (1976), J. Bacteriol., 127, 1197-1207.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.5.1.34 NADH protects against inactivation Pseudomonas sp.
1.5.1.34 NADPH protects against inactivation Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.34 5,5'-dithiobis(2-nitrobenzoate) 0.57 mM, 98% inhibition Pseudomonas sp.
1.5.1.34 aminopterin competitive vs. 2-amino-4-hydroxy-6,7-dimethyl-quinonoid-dihydropteridine Pseudomonas sp.
1.5.1.34 CdCl2 0.1 mM, complete inactivation Pseudomonas sp.
1.5.1.34 HgCl2
-
 Pseudomonas sp.
1.5.1.34 MgCl2 0.1 mM, complete inactivation Pseudomonas sp.
1.5.1.34 MnCl2 0.1 mM, complete inactivation Pseudomonas sp.
1.5.1.34 N-ethylmaleimide 5 mM, complete inhibition of reduced enzyme Pseudomonas sp.
1.5.1.34 p-chloromercuribenzoate 0.4 mM, 50% inhibition Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.34 0.16
-
 5,6,7,8-tetrahydrobiopterin
-
 Pseudomonas sp.
1.5.1.34 0.16
-
 quinonoid dihydrobiopterin
-
 Pseudomonas sp.
1.5.1.34 0.34
-
 quinonoid 2-amino-4-hydroxy-6,7-dimethyldihydropteridine
-
 Pseudomonas sp.
1.5.1.34 0.34
-
 2-Amino-4-hydroxy-6,7-dimethyl-quinonoid-dihydropteridine
-
 Pseudomonas sp.
1.5.1.34 1.45
-
 L-threo-neopterin
-
 Pseudomonas sp.
1.5.1.34 1.45
-
 quinonoid L-threo-dihydroneopterin
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.34 43500 49000 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.34 quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H Pseudomonas sp. no marked specificity for the pteridine cofactor that occurs naturally in this organism L-threo-neopterin 5,6,7,8-tetrahydrobiopterin + NAD(P)+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.34 Pseudomonas sp.
-
 sp. ATCC 11299a
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.34 ATCC 11299a Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.34 33.2
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
1.5.1.34 -20°C, stable for at least a year, enzyme activity in ammonium sulfate precipitate Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.34 2-amino-4-hydroxy-6,7-dimethyl-quinonoid-dihydropteridine + NAD(P)H 3% activity with NADPH Pseudomonas sp. 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine + NAD(P)+
-
 ?
1.5.1.34 5,6,7,8-tetrahydrobiopterin + NAD+
-
 Pseudomonas sp. quinonoid dihydrobiopterin + NADH + H+
-
 r
1.5.1.34 L-threo-neopterin + NAD+
-
 Pseudomonas sp. ?
-
 r
1.5.1.34 quinoid dihydrobiopterin + NAD(P)H specific for NADH Pseudomonas sp. tetrahydrobiopterin + NAD(P)+
-
 ?
1.5.1.34 quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H no marked specificity for the pteridine cofactor that occurs naturally in this organism L-threo-neopterin Pseudomonas sp. 5,6,7,8-tetrahydrobiopterin + NAD(P)+
-
 ?
1.5.1.34 quinonoid dihydro-L-threo-neopterin + NAD(P)H
-
 Pseudomonas sp. tetrahydro-L-threo-neopterin + NAD(P)+
-
 ?
1.5.1.34 quinonoid dihydrobiopterin + NADH + H+
-
 Pseudomonas sp. 6R-(1'R,2'R)-5,6,7,8-tetrahydrobiopterin + NAD+
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.1.34 40
-
 5 min, 80% loss of activity, EDTA protects Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.34 20
-
 quinonoid dihydrobiopterin
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.34 7.2
-
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.34 NADH enzyme prefers NADH Pseudomonas sp.
1.5.1.34 NADPH NADH is preferred Pseudomonas sp.
1.5.1.34 NADPH inactive with NADPH, specific for NADPH Pseudomonas sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.34 0.21
-
 aminopterin
-
 Pseudomonas sp.