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Literature summary extracted from

  • Hasegawa, H.; Nakanishi, N.
    Dihydropteridine reductase from bovine liver (1987), Methods Enzymol., 142, 103-110.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.34 aminopterin 0.025 mM, 50% inhibition of NADH-specific enzyme, 1 mM, 50% inhibition of NADPH-specific enzyme Bos taurus
1.5.1.34 methopterin 0.038 mM, 50% inhibition of NADH-specific enzyme Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.34 0.00079
-
 NADH reduction of quinonoid 7,8-dihydro-6-methylpterin Bos taurus
1.5.1.34 0.0011
-
 quinonoid 7,8-dihydrobiopterin cofactor NADPH Bos taurus
1.5.1.34 0.0015
-
 NADH reduction of quinonoid 7,8-dihydrobiopterin Bos taurus
1.5.1.34 0.0016
-
 quinonoid 7,8-dihydrobiopterin cofactor NADH Bos taurus
1.5.1.34 0.0056
-
 quinonoid 7,8-dihydro-6-methylpterin cofactor NADH Bos taurus
1.5.1.34 0.0058
-
 quinonoid 7,8-dihydro-6-methylpterin cofactor NADPH Bos taurus
1.5.1.34 0.074
-
 NADPH reduction of quinonoid 7,8-dihydrobiopterin Bos taurus
1.5.1.34 0.095
-
 NADPH reduction of quinonoid 7,8-dihydrobiopterin Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.34 25000
-
 2 * 25000, NADH-dihydropteridine reductase Bos taurus
1.5.1.34 49000 50000 NADH-dihydropteridine reductase Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.34 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.34
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.34 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.34 70.7
-
 NADH-specific enzyme Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.34 quinonoid 6-methyl-7,8-(6H)-dihydropterin + NAD(P)H
-
 Bos taurus 6-methyl-5,6,7,8-tetrahydropterin + NAD(P)+
-
 ?
1.5.1.34 quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H
-
 Bos taurus 5,6,7,8-tetrahydrobiopterin + NAD(P)+
-
 ?
1.5.1.34 quinonoid dihydrobiopterin + NADH + H+
-
 Bos taurus 6R-(1'R,2'R)-5,6,7,8-tetrahydrobiopterin + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.34 dimer 2 * 25000, NADH-dihydropteridine reductase Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.34 NADH utilizes both NADH and NADPH Bos taurus
1.5.1.34 NADH enzyme prefers NADH Bos taurus
1.5.1.34 NADH liver: 2 distinct dihydropteridine reductases which catalyze the conversion of the quinonoid dihydropteridine to tetrahydropterin: NADH-dihydropteridine reductase, DPR, utilizes NADH as a better substrate than NADPH, NADPH-specific dihydropteridine reductase, TPR, shows strict specificity for NADPH Bos taurus
1.5.1.34 NADPH utilizes both NADH and NADPH Bos taurus
1.5.1.34 NADPH liver: 2 distinct dihydropteridine reductases which catalyze the conversion of the quinonoid dihydropteridine to tetrahydropterin: NADH-dihydropteridine reductase, DPR, utilizes NADH as a better substrate than NADPH, NADPH-specific dihydropteridine reductase, TPR, shows strict specificity for NADPH Bos taurus
1.5.1.34 NADPH NADH is preferred Bos taurus