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Literature summary extracted from

  • Kaufman, S.
    Dihydropteridine reductase from sheep liver (1987), Methods Enzymol., 142, 97-103.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.34 aminopterin
-
 Ovis aries
1.5.1.34 methotrexate
-
 Ovis aries

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.34 0.001
-
 quinonoid dihydrobiopterin cofactor NADH Ovis aries
1.5.1.34 0.004
-
 NADH reduction of quinonoid dihydrobiopterin Ovis aries
1.5.1.34 0.0057
-
 NADH reduction of quinonoid 2-amino-4-hydroxy-6,7-dimetyldihydropteridine Ovis aries
1.5.1.34 0.006
-
 quinonoid 2-amino-4-hydroxy-6,7-dimethyldihydropteridine cofactor NADPH Ovis aries
1.5.1.34 0.009
-
 quinonoid dihydrobiopterin cofactor NADPH Ovis aries
1.5.1.34 0.0152
-
 2-amino-4-hydroxy-6,7-dimethyldihydropteridine cofactor NADH Ovis aries
1.5.1.34 0.0709
-
 NADPH reduction of quinonoid dihydrobiopterin Ovis aries
1.5.1.34 0.08
-
 NADPH reduction of quinonoid 2-amino-4-hydroxy-6,7-dimethyldihydropteridine Ovis aries

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.34 21000
-
 2 * 21000, liver, SDS-PAGE Ovis aries
1.5.1.34 41000
-
 gradient PAGE Ovis aries

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.34 quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H Ovis aries essential component of the hepatic phenylalanine hydroxylating system 5,6,7,8-tetrahydrobiopterin + NAD(P)+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.34 Ovis aries
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.34
-
 Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.34 adrenal medulla
-
 Ovis aries
-
1.5.1.34 brain
-
 Ovis aries
-
1.5.1.34 heart low activity Ovis aries
-
1.5.1.34 kidney
-
 Ovis aries
-
1.5.1.34 liver
-
 Ovis aries
-
1.5.1.34 skeletal muscle low activity Ovis aries
-
1.5.1.34 spleen low activity Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.34 15.6
-
-
 Ovis aries

Storage Stability

EC Number Storage Stability Organism
1.5.1.34 -80°C, stable for years Ovis aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.34 2-amino-4-hydroxy-6,7-dimethyl-quinonoid-dihydropteridine + NAD(P)H
-
 Ovis aries 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine + NAD(P)+
-
 ?
1.5.1.34 quinonoid 6,7-dimethyl-7,8-(6H)-dihydropterin + NAD(P)H
-
 Ovis aries 6,7-dimethyl-5,6,7,8-tetrahydropterin + NAD(P)+
-
 ?
1.5.1.34 quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H absolutely specific for quinonoid dihydro-isomer corresponding to 7,8-dihydropterins Ovis aries 5,6,7,8-tetrahydrobiopterin + NAD(P)+
-
 ?
1.5.1.34 quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H essential component of the hepatic phenylalanine hydroxylating system Ovis aries 5,6,7,8-tetrahydrobiopterin + NAD(P)+
-
 ?
1.5.1.34 quinonoid dihydro-2-methylamino-4-hydroxy-6,7-dimethylpteridine + NAD(P)H approx. one-third of activity with 2-amino-4-hydroxy-6,7-dimethyl-quinonoid-dihydropterid Ovis aries tetrahydro-2-methylamino-4-hydroxy-6,7-dimethylpteridine + NAD(P)+
-
 ?
1.5.1.34 quinonoid dihydrobiopterin + NADH + H+
-
 Ovis aries 6R-(1'R,2'R)-5,6,7,8-tetrahydrobiopterin + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.34 dimer 2 * 21000, liver, SDS-PAGE Ovis aries

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.34 6.8 7.8 pH 6.8: about 45% activity, pH 7.8: about 15% activity Ovis aries

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.34 NADH utilizes both NADH and NADPH Ovis aries
1.5.1.34 NADH enzyme prefers NADH Ovis aries
1.5.1.34 NADPH utilizes both NADH and NADPH Ovis aries
1.5.1.34 NADPH NADH is preferred Ovis aries

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.34 0.038
-
 methotrexate
-
 Ovis aries