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Literature summary extracted from

  • Robello, C.; Navarro, P.; Castanys, S.; Gamarro, F.
    A pteridine reductase gene ptr1 contiguous to a P-glycoprotein confers resistance to antifolates in Trypanosoma cruzi (1997), Mol. Biochem. Parasitol., 90, 525-535.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.33
-
Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.33 30000
-
x * 30000, calculation from nucleotide sequence Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.33 additional information Trypanosoma cruzi the enzyme is involved in the resistance to the methotrexate, aminopterin and trimethoprim antifolates ?
-
?
1.5.1.33 additional information Trypanosoma cruzi Y the enzyme is involved in the resistance to the methotrexate, aminopterin and trimethoprim antifolates ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.33 Trypanosoma cruzi
-
Y strain
-
1.5.1.33 Trypanosoma cruzi Y
-
Y strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.33 recombinant enzyme Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.33 Biopterin + NADPH no activity with NADH and NADP+ Trypanosoma cruzi 5,6,7,8-Tetrahydrobiopterin + NADP+
-
?
1.5.1.33 Biopterin + NADPH no activity with NADH and NADP+ Trypanosoma cruzi Y 5,6,7,8-Tetrahydrobiopterin + NADP+
-
?
1.5.1.33 Dihydrobiopterin + NADPH
-
Trypanosoma cruzi ?
-
?
1.5.1.33 Dihydrobiopterin + NADPH
-
Trypanosoma cruzi Y ?
-
?
1.5.1.33 Dihydrofolate + NADPH
-
Trypanosoma cruzi 5,6,7,8-Tetrahydrofolate + NADP+
-
?
1.5.1.33 Dihydrofolate + NADPH
-
Trypanosoma cruzi Y 5,6,7,8-Tetrahydrofolate + NADP+
-
?
1.5.1.33 Dihydroneopterin + NADPH no activity Trypanosoma cruzi ?
-
?
1.5.1.33 dihydrosepiapterin + NADPH no activity Trypanosoma cruzi ?
-
?
1.5.1.33 folate + NADPH
-
Trypanosoma cruzi 5,6,7,8-tetrahydrofolate + NADP+
-
?
1.5.1.33 folate + NADPH
-
Trypanosoma cruzi Y 5,6,7,8-tetrahydrofolate + NADP+
-
?
1.5.1.33 additional information the enzyme is involved in the resistance to the methotrexate, aminopterin and trimethoprim antifolates Trypanosoma cruzi ?
-
?
1.5.1.33 additional information the enzyme is involved in the resistance to the methotrexate, aminopterin and trimethoprim antifolates Trypanosoma cruzi Y ?
-
?

Subunits

EC Number Subunits Comment Organism
1.5.1.33 ? x * 30000, calculation from nucleotide sequence Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.33 6
-
biopterin as substrate Trypanosoma cruzi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.5.1.33 4.7 7 significant decline of activity at pH 7 and 4.7 Trypanosoma cruzi

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.33 NADPH dependent on Trypanosoma cruzi