Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kabisch, U.C.; Grantzdorffer, A.; Schierhorn, A.; Rucknagel, K.P.; Andreesen, J.R.; Pich, A.
    Identification of D-proline reductase from Clostridium sticklandii as a selenoenzyme and indications for a catalytically active pyruvoyl group derived from a cysteine residue by cleavage of a proprotein (1999), J. Biol. Chem., 274, 8445-8454.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.21.4.1 cloning of the genes encoding D-proline reductase, 4.8 kb EcoRI fragment containing the genes prdA and prdB isolated and sequenced, prdAcodes for a 68 kDa protein, posttranslationally cleaved to the 45 kDa and 23 kDa subunits, prdB encodes the 26 kDa subunit Acetoanaerobium sticklandii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.21.4.1 cytoplasm
-
 Acetoanaerobium sticklandii 5737
-
1.21.4.1 membrane
-
 Acetoanaerobium sticklandii 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.21.4.1 Mg2+ essential for the reaction Acetoanaerobium sticklandii
1.21.4.1 Se2+ containing selenium in form of selenocysteine and a carbonyl moiety, most probably a pyruvoyl group Acetoanaerobium sticklandii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.21.4.1 23000
-
 10 * 23000 + 10 * 26000+ 10 * 45000, SDS-PAGE, mass spectrometry Acetoanaerobium sticklandii
1.21.4.1 30000
-
 10 * 30000 Acetoanaerobium sticklandii
1.21.4.1 45000
-
 10 * 23000 + 10 * 26000+ 10 * 45000, SDS-PAGE, mass spectrometry Acetoanaerobium sticklandii
1.21.4.1 300000
-
 gel filtration Acetoanaerobium sticklandii
1.21.4.1 870000
-
 gel filtration, native PAGE Acetoanaerobium sticklandii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.4.1 D-proline + NADH Acetoanaerobium sticklandii NADH is more effective than dithiols in crude preparations 5-aminopentanoic acid + NAD+
-
 ?
1.21.4.1 D-proline + NADH Acetoanaerobium sticklandii DSM 519 NADH is more effective than dithiols in crude preparations 5-aminopentanoic acid + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.4.1 Acetoanaerobium sticklandii
-
 strain HF, DSM 517 T
-
1.21.4.1 Acetoanaerobium sticklandii DSM 519
-
 strain HF, DSM 517 T
-
1.21.4.1 Clostridium sporogenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.21.4.1
-
 Acetoanaerobium sticklandii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.21.4.1 16.1
-
-
 Acetoanaerobium sticklandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.4.1 D-proline + dithiol (reduced)
-
 Acetoanaerobium sticklandii 5-aminopentanoic acid + oxidized dithiol
-
 ?
1.21.4.1 D-proline + dithiol (reduced)
-
 Acetoanaerobium sticklandii DSM 519 5-aminopentanoic acid + oxidized dithiol
-
 ?
1.21.4.1 D-proline + dithiothreitol dithiothreitol as artificial electron donor Acetoanaerobium sticklandii 5-amino pentanoic acid + ?
-
 ir
1.21.4.1 D-proline + dithiothreitol Stickland reaction Acetoanaerobium sticklandii 5-amino pentanoic acid + ?
-
 ir
1.21.4.1 D-proline + dithiothreitol dithiothreitol as artificial electron donor Acetoanaerobium sticklandii DSM 519 5-amino pentanoic acid + ?
-
 ir
1.21.4.1 D-proline + dithiothreitol Stickland reaction Acetoanaerobium sticklandii DSM 519 5-amino pentanoic acid + ?
-
 ir
1.21.4.1 D-proline + NADH NADH is more effective than dithiols in crude preparations Acetoanaerobium sticklandii 5-aminopentanoic acid + NAD+
-
 ?
1.21.4.1 D-proline + NADH NADH is more effective than dithiols in crude preparations Acetoanaerobium sticklandii DSM 519 5-aminopentanoic acid + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.21.4.1 decamer 10 * 30000 Acetoanaerobium sticklandii
1.21.4.1 decamer 10 * 23000 + 10 * 26000+ 10 * 45000, SDS-PAGE, mass spectrometry Acetoanaerobium sticklandii

Synonyms

EC Number Synonyms Comment Organism
1.21.4.1 EC 1.4.1.6 formerly Acetoanaerobium sticklandii