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Literature summary extracted from

  • Danneel, H.J.; Reichert, A.; Giffhorn, F.
    Production, purification and characterization of an alcohol oxidase of the ligninolytic fungus Peniophora gigantea (1994), J. Biotechnol., 33, 33-41.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.1.3.13 rapid inactivation by repeated freezing and thawing Phlebiopsis gigantea

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.3.13 Cu2+ 1 mM CuCl2, complete inhibition Phlebiopsis gigantea
1.1.3.13 FeSO4 1 mM, complete inhibition Phlebiopsis gigantea
1.1.3.13 NiCl2 1 mM, complete inhibition Phlebiopsis gigantea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.13 0.4
-
 O2
-
 Phlebiopsis gigantea
1.1.3.13 1.8
-
 methanol
-
 Phlebiopsis gigantea
1.1.3.13 2.9
-
 ethanol
-
 Phlebiopsis gigantea
1.1.3.13 71.5
-
 D-ribose
-
 Phlebiopsis gigantea
1.1.3.13 250
-
 ribitol
-
 Phlebiopsis gigantea
1.1.3.13 670
-
 erythritol
-
 Phlebiopsis gigantea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.13 72500
-
 8 * 72500, SDS-PAGE Phlebiopsis gigantea
1.1.3.13 576000
-
 gel filtration Phlebiopsis gigantea

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.13 Phlebiopsis gigantea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.13
-
 Phlebiopsis gigantea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.3.13 mycelium produced in the stationary growth phase Phlebiopsis gigantea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.13 8.7
-
-
 Phlebiopsis gigantea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.13 allylalcohol + O2
-
 Phlebiopsis gigantea acrolein + H2O2
-
 ?
1.1.3.13 D-ribose + O2
-
 Phlebiopsis gigantea ? + H2O2
-
 ?
1.1.3.13 erythritol + O2
-
 Phlebiopsis gigantea ? + H2O2
-
 ?
1.1.3.13 ethanol + O2
-
 Phlebiopsis gigantea acetaldehyde + H2O2
-
 ?
1.1.3.13 isopropanol + O2
-
 Phlebiopsis gigantea 2-propanone + H2O2
-
 ?
1.1.3.13 methanol + O2
-
 Phlebiopsis gigantea formaldehyde + H2O2
-
 ?
1.1.3.13 n-butanol + O2
-
 Phlebiopsis gigantea n-butanal + H2O2
-
 ?
1.1.3.13 n-propanol + O2
-
 Phlebiopsis gigantea n-propanal + H2O2
-
 ?
1.1.3.13 ribitol + O2
-
 Phlebiopsis gigantea ? + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.13 octamer 8 * 72500, SDS-PAGE Phlebiopsis gigantea

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.3.13 22
-
 50% loss of activity after 10 min in 0.1 M potassium phosphate buffer, pH 6.0. 50% loss of activity after 60 min in 0.1 M potassium phosphate buffer, pH 7.0, 50% loss of activity after 28 h in 0.1 M Tris-HCl buffer, pH 8.0. 50% loss of activity after 16 h in 0.1 M Tris-HCl buffer, pH 9.0 Phlebiopsis gigantea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.13 7.3 9
-
 Phlebiopsis gigantea

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.3.13 6
-
 22°C, 50% loss of activity after 10 min Phlebiopsis gigantea
1.1.3.13 7
-
 22°C, 50% loss of activity after 60 min Phlebiopsis gigantea
1.1.3.13 8
-
 22°C, 50% loss of activity after 28 h Phlebiopsis gigantea
1.1.3.13 9
-
 22°C, 50% loss of activity after 10 min Phlebiopsis gigantea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.13 FAD covalently bound Phlebiopsis gigantea
1.1.3.13 FAD 1 mol FAD per mol of subunit Phlebiopsis gigantea