EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.3.13 | - |
Pichia sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.13 | 1,10-phenanthroline | 1 mM, 10% inhibition | Pichia sp. | |
1.1.3.13 | 2,2'-dipyridyl | 1 mM, 13% inhibition | Pichia sp. | |
1.1.3.13 | 2-Aminoethanol | 1 mM, 70% inhibition | Pichia sp. | |
1.1.3.13 | 3-Pentanol | 1 mM, 16% inhibition | Pichia sp. | |
1.1.3.13 | 5,5'-dithiobis(2-nitrobenzoate) | 1 mM, 35% inhibition | Pichia sp. | |
1.1.3.13 | 8-hydroxyquinoline | 1 mM, 6% inhibition | Pichia sp. | |
1.1.3.13 | acetamide | 1 mM, 10% inhibition | Pichia sp. | |
1.1.3.13 | Cu2+ | 1 mM CuSO4, 50% inhibition | Pichia sp. | |
1.1.3.13 | H2O2 | - |
Pichia sp. | |
1.1.3.13 | hydroxylamine | 1 mM, 21% inhibition | Pichia sp. | |
1.1.3.13 | imidazole | 1 mM, 20% inhibition | Pichia sp. | |
1.1.3.13 | iodoacetate | 1 mM, 38% inhibition | Pichia sp. | |
1.1.3.13 | Mercuric chloride | 1 mM, complete inhibition | Pichia sp. | |
1.1.3.13 | metal chelators | slight | Pichia sp. | |
1.1.3.13 | NEM | 1 mM, 10% inhibition | Pichia sp. | |
1.1.3.13 | p-hydroxymercuribenzoate | 1 mM, complete inhibition | Pichia sp. | |
1.1.3.13 | PCMB | - |
Pichia sp. | |
1.1.3.13 | Sodium azide | 1 mM, 15% inhibition | Pichia sp. | |
1.1.3.13 | Thiosemicarbazide | 1 mM, 31% loss of activity | Pichia sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.3.13 | 0.5 | - |
methanol | - |
Pichia sp. | |
1.1.3.13 | 3.5 | - |
formaldehyde | - |
Pichia sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.3.13 | 76000 | - |
4 * 76000, SDS-PAGE | Pichia sp. |
1.1.3.13 | 300000 | - |
gel filtration | Pichia sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.13 | methanol + O2 | Pichia sp. | key enzyme of methanol metabolism | formaldehyde + H2O2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.13 | Pichia sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.3.13 | - |
Pichia sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.3.13 | 6.6 | - |
- |
Pichia sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.13 | 1-pentanol + O2 | 30% of the activity with methanol | Pichia sp. | n-pentanal + H2O2 | - |
? | |
1.1.3.13 | 2-mercaptoethanol + O2 | 25% of the activity with methanol | Pichia sp. | 2-mercaptoethanal + H2O2 | - |
? | |
1.1.3.13 | 2-methyl-1-butanol + O2 | 22% of the activity with methanol | Pichia sp. | 2-methyl-1-butanal + H2O2 | - |
? | |
1.1.3.13 | 3-chloro-1-propanol + O2 | 22% of the activity with methanol | Pichia sp. | 3-chloro-1-propanal + H2O2 | - |
? | |
1.1.3.13 | 4-chloro-1-butanol + O2 | 11% of the activity with methanol | Pichia sp. | 4-chloro-1-butanal + H2O2 | - |
? | |
1.1.3.13 | chloroethanol + O2 | 2-chloroethanol, 70% of the activity with methanol | Pichia sp. | chloroethanal + H2O2 | - |
? | |
1.1.3.13 | ethanol + O2 | 92% of the activity with methanol | Pichia sp. | acetaldehyde + H2O2 | - |
? | |
1.1.3.13 | ethylene glycol mono-methylether + O2 | - |
Pichia sp. | methoxyacetaldehyde + H2O2 | - |
? | |
1.1.3.13 | formaldehyde + O2 | 15% of the activity with methanol | Pichia sp. | formate + H2O2 | - |
? | |
1.1.3.13 | isobutanol + O2 | 2% of the activity with methanol | Pichia sp. | isobutanal + H2O2 | - |
? | |
1.1.3.13 | methanol + O2 | - |
Pichia sp. | formaldehyde + H2O2 | - |
? | |
1.1.3.13 | methanol + O2 | key enzyme of methanol metabolism | Pichia sp. | formaldehyde + H2O2 | - |
? | |
1.1.3.13 | n-butanol + O2 | 52%, of the activity with methanol | Pichia sp. | n-butanal + H2O2 | - |
? | |
1.1.3.13 | n-hexanol + O2 | 4% of the activity with methanol | Pichia sp. | n-hexanal + H2O2 | - |
? | |
1.1.3.13 | n-propanol + O2 | - |
Pichia sp. | n-propanal + H2O2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.3.13 | tetramer | 4 * 76000, SDS-PAGE | Pichia sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.13 | 9 | - |
- |
Pichia sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.3.13 | 6 | 10 | pH 6.0: about 65% of maximal activity, pH 10.0: 85% of maximal activity | Pichia sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.3.13 | FAD | - |
Pichia sp. |