Literature summary extracted from

Webster, J.J.; Chang, J.C.; Manley, E.R.; Spivey, H.O.; Leach ,F.R.
Buffer effects on ATP analysis by firefly luciferase (1980), Anal. Biochem., 106, 7-11.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.13.12.7	arsenate	-	Photinus pyralis

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.13.12.7	0.042	-	Luciferin	phosphate buffer	Photinus pyralis
1.13.12.7	0.055	-	Luciferin	glycylglycine buffer	Photinus pyralis
1.13.12.7	0.125	-	ATP	tricine buffer	Photinus pyralis
1.13.12.7	0.2	-	ATP	phosphate buffer	Photinus pyralis
1.13.12.7	0.285	-	ATP	glycylglycine buffer	Photinus pyralis

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.13.12.7	Photinus pyralis	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.13.12.7	Photinus luciferin + O2 + ATP	-	Photinus pyralis	oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv	-	?

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.13.12.7	7.6	-	tricine buffer	Photinus pyralis

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Release 2023.1 (January 2023)