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Literature summary extracted from

  • Webster, J.J.; Leach, F.R.
    Optimization of the firefly luciferase assay for ATP (1980), J. Appl. Biochem., 2, 469-479.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.12.7 Luciferin preparations supplemented with extra luciferin provide maximum light emission Photinus pyralis

General Stability

EC Number General Stability Organism
1.13.12.7 bovine serum albumin stabilizes Photinus pyralis
1.13.12.7 dithiothreitol stabilizes Photinus pyralis
1.13.12.7 EDTA stabilizes Photinus pyralis
1.13.12.7 more stable in phosphate buffer than in Tricine buffer Photinus pyralis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.12.7 dithiothreitol
-
 Photinus pyralis
1.13.12.7 EDTA
-
 Photinus pyralis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.12.7 Cu2+
-
 Photinus pyralis
1.13.12.7 Mg2+ concentration 5 mM, MgSO4, MgCl2, Mg(O2CCH3)2 equally suitable salts Photinus pyralis

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.7 Photinus pyralis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.7 Photinus luciferin + O2 + ATP the true substrate is MgATP2- Photinus pyralis oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.12.7 25
-
-
 Photinus pyralis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.7 7.8
-
-
 Photinus pyralis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.12.7 additional information
-
 buffers with pKa from 7.2 to 8.4 tested and no relationship with activity found Photinus pyralis
1.13.12.7 6.5 8.5 pH 6.5: about 45% of activity maximum, pH 8.5: about 35% of activity maximum Photinus pyralis