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Literature summary extracted from

  • Rajgopal S; Vijayalakshmi M.A.
    Firefly luciferase: purification and immobilization (1984), Enzyme Microb. Technol., 6, 482-490.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.13.12.7 extraction, 2 gel elutions, 5 crystallizations Photinus pyralis

General Stability

EC Number General Stability Organism
1.13.12.7 dithiothreitol stabilizes Photinus pyralis
1.13.12.7 stability increases 1000fold with immobilization on CNBr-Sepharose 4B, pH 7.5 at 25°C Luciola mingrelica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.12.7 100000
-
 from sedimentation velocity of the crystalline form Photinus pyralis

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.7 Luciola mingrelica
-
-
-
1.13.12.7 Photinus pyralis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.7 chromatography on DEAE-cellulose Luciola mingrelica
1.13.12.7 several chromatographic and non chromatographic methods Photinus pyralis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.12.7 additional information
-
-
 Photinus pyralis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.7 Photinus luciferin + O2 + ATP
-
 Photinus pyralis oxidized Photinus luciferin + CO2 + H2O + AMP + diphosphate + hv
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.12.7 dimer 1 * alpha (catalytic subunit) + 1 * beta Photinus pyralis
1.13.12.7 dimer two non identical subunits Photinus pyralis