EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.6 | Mg2+ | synthesis of lysyl-tRNA from lysyl-AMP-enzyme inhibited, formation of Lys-tRNALys from lysine, tRNA and ATP-enzyme complex or ATP is dependent on Mg2+ | Escherichia coli | |
6.1.1.6 | NH4+ | formation of Lys-tRNALys from lysine, tRNA and ATP-enzyme complex or ATP | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.6 | 0.004 | - |
Lys | aminoacylation | Escherichia coli | |
6.1.1.6 | 0.005 | - |
L-Lys | aminoacylation, enzyme form EII | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.6 | Mg2+ | formation of Lys-tRNALys from lysine, tRNA and ATP-enzyme complex or ATP is dependent on Mg2+ | Escherichia coli | |
6.1.1.6 | NH4+ | synthesis of lysyl-tRNA from lysyl-AMP-enzyme is dependent on NH4+ or other monovalent cations | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.1.1.6 | 72000 | - |
1 * 72000 + 1 + 62000, SDS-PAGE | Escherichia coli |
6.1.1.6 | 150000 | - |
gel filtration | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.6 | Escherichia coli | - |
B | - |
6.1.1.6 | Escherichia coli B / ATCC 11303 | - |
B | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
6.1.1.6 | - |
Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.1.1.6 | 19.6 | - |
ATP-diphosphate exchange | Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
6.1.1.6 | -15°C, 2 years | Escherichia coli |
6.1.1.6 | 4°C, 8 months | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.6 | ATP + lysine + tRNALys | - |
Escherichia coli | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
6.1.1.6 | ATP + lysine + tRNALys | - |
Escherichia coli B / ATCC 11303 | AMP + L-lysyl-tRNALys + diphosphate | - |
? | |
6.1.1.6 | additional information | lysine-dependent ATP-diphosphate exchange reaction: lysine + ATP + enzyme/lysine-AMP-enzyme + diphosphate | Escherichia coli | ? | - |
? | |
6.1.1.6 | additional information | lysine-dependent ATP-diphosphate exchange reaction: lysine + ATP + enzyme/lysine-AMP-enzyme + diphosphate | Escherichia coli B / ATCC 11303 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.1.1.6 | dimer | 1 * 72000 + 1 + 62000, SDS-PAGE | Escherichia coli |