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Literature summary extracted from

  • Weiss, P.M.; Garcia, G.A.; Kenyon, G.L.; Cleland, W.W.; Cook, P.F.
    Kinetics and mechanism of benzoylformate decarboxylase using 13C and solvent deuterium isotope effects on benzoylformate and benzoylformate analogues (1988), Biochemistry, 27, 2197-2205.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.7 (R)-mandelate competitive Pseudomonas putida
4.1.1.7 (S)-Mandelate competitive Pseudomonas putida
4.1.1.7 2-carboxybenzaldehyde competitive Pseudomonas putida
4.1.1.7 Phenoxyacetate competitive Pseudomonas putida
4.1.1.7 phenylacetate competitive Pseudomonas putida
4.1.1.7 Phthalate competitive Pseudomonas putida
4.1.1.7 Phthalimide competitive Pseudomonas putida
4.1.1.7 trans-cinnamate competitive Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.7 0.056
-
 (p-methoxybenzoyl)formate
-
 Pseudomonas putida
4.1.1.7 0.059
-
 benzoylformate
-
 Pseudomonas putida
4.1.1.7 0.18
-
 [p-(Chloromethyl)benzoyl]formate
-
 Pseudomonas putida
4.1.1.7 0.19
-
 (p-methylbenzoyl)formate
-
 Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.7 Pseudomonas putida
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.1.7 phenylglyoxylate = benzaldehyde + CO2 the reaction has two partially rate-determining steps: initial tetrahedral adduct formation and decarboxylation Pseudomonas putida
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.7 (p-methoxybenzoyl)formate
-
 Pseudomonas putida p-methoxybenzaldehyde + CO2
-
 ?
4.1.1.7 (p-methylbenzoyl)formate
-
 Pseudomonas putida p-methylbenzaldehyde + CO2
-
 ?
4.1.1.7 benzoylformate
-
 Pseudomonas putida benzaldehyde + CO2
-
 ?
4.1.1.7 [m-(Fluoromethyl)benzoyl]formate
-
 Pseudomonas putida ?
-
 ?
4.1.1.7 [p-(Chloromethyl)benzoyl]formate
-
 Pseudomonas putida ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.7 thiamine diphosphate dependent on Pseudomonas putida