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Literature summary extracted from

  • Reynolds, L.J.; Garcia, G.A.; Kozarich, J.W.; Kenyon, G.L.
    Differential reactivity in the processing of [p-(halomethyl)benzoyl]formates by benzoylformate decarboxylase, a thiamin pyrophosphate dependent enzyme (1988), Biochemistry, 27, 5530-5538.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.7 [p-(Bromomethyl)benzoyl]formate due to covalent modification of the cofactor Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.7 0.021
-
 [p-(Chloromethyl)benzoyl]formate
-
 Pseudomonas putida
4.1.1.7 0.023
-
 [m-(fluoromethyl)benzoyl]formate
-
 Pseudomonas putida
4.1.1.7 0.19
-
 [p-(Fluoromethyl)benzoyl]formate
-
 Pseudomonas putida
4.1.1.7 0.34
-
 benzoylformate
-
 Pseudomonas putida
4.1.1.7 0.58
-
 (p-methylbenzoyl)formate
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.7 80000
-
 gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.7 Pseudomonas putida
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.7 193
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.7 (p-methylbenzoyl)formate
-
 Pseudomonas putida p-methylbenzaldehyde + CO2
-
 ?
4.1.1.7 benzoylformate
-
 Pseudomonas putida benzaldehyde + CO2
-
 ?
4.1.1.7 [p-(Chloromethyl)benzoyl]formate
-
 Pseudomonas putida ?
-
 ?
4.1.1.7 [p-(Fluoromethyl)benzoyl]formate
-
 Pseudomonas putida ?
-
 ?