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Literature summary extracted from
- Purification, nucleotide sequence and some properties of a bifunctional isomerase/decarboxylase from the homoprotocatechuate degradative pathway of Escherichia coli C (1993), Eur. J. Biochem., 217, 575-580.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.1.68 | - |
Escherichia coli |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.1.68 | 0.032 | - |
5-oxopent-3-ene-1,2,5-tricarboxylate | - |
Escherichia coli |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.68 | 27000 | - |
gel filtration | Escherichia coli |
| 4.1.1.68 | 42100 | - |
sedimentation equilibrium centrifugation | Escherichia coli |
| 4.1.1.68 | 44514 | - |
1 * 44514, calculation from nucleotide sequence | Escherichia coli |
| 4.1.1.68 | 50000 | - |
1 * 50000, SDS-PAGE | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.1.68 | Escherichia coli | - |
- |
- |
| 4.1.1.68 | Escherichia coli | - |
bifunctional enzyme with activity of EC 5.3.3.10 and EC 4.1.1.68 | - |
| 4.1.1.68 | Escherichia coli C | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.1.68 | - |
Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.68 | 45 | - |
- |
Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.1.1.68 | 4°C, stable for at least 2 months | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.68 | 5-Oxopent-3-ene-1,2,5-tricarboxylate | - |
Escherichia coli | 2-Oxohept-3-enedioate + CO2 | - |
? | |
| 4.1.1.68 | 5-Oxopent-3-ene-1,2,5-tricarboxylate | - |
Escherichia coli C | 2-Oxohept-3-enedioate + CO2 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.1.68 | monomer | 1 * 50000, SDS-PAGE | Escherichia coli |
| 4.1.1.68 | monomer | 1 * 44514, calculation from nucleotide sequence | Escherichia coli |
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