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Literature summary extracted from

  • Gallert, C.; Winter, J.
    Comparison of 4-hydroxybenzoate decarboxylase, and phenol carboxylase activities in cell-free extracts of a defined 4-hydroxybenzoate and phenol-degrading anaerobic consortium (1992), Appl. Microbiol. Biotechnol., 37, 119-124.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.61 2,3,4-Trihydroxybenzoate complete inhibition of carboxylation of phenol Desulfovibrio sp.
4.1.1.61 2,3,4-Trihydroxybenzoate complete inhibition of carboxylation of phenol Methanospirillum hungatei
4.1.1.61 2,4,6-Trihydroxybenzoate complete inhibition of carboxylation of phenol Desulfovibrio sp.
4.1.1.61 2,4,6-Trihydroxybenzoate complete inhibition of carboxylation of phenol Methanospirillum hungatei
4.1.1.61 2,4-Dihydroxybenzoate
-
 Desulfovibrio sp.
4.1.1.61 2,4-Dihydroxybenzoate
-
 Methanospirillum hungatei
4.1.1.61 3,4,5-Trihydroxybenzoate slight inhibition of carboxylation of phenol Desulfovibrio sp.
4.1.1.61 3,4,5-Trihydroxybenzoate slight inhibition of carboxylation of phenol Methanospirillum hungatei
4.1.1.61 3-Chloro-4-hydroxybenzoate
-
 Desulfovibrio sp.
4.1.1.61 3-Chloro-4-hydroxybenzoate
-
 Methanospirillum hungatei
4.1.1.61 Co2+
-
 Desulfovibrio sp.
4.1.1.61 Co2+
-
 Methanospirillum hungatei
4.1.1.61 EDTA
-
 Desulfovibrio sp.
4.1.1.61 EDTA
-
 Methanospirillum hungatei
4.1.1.61 m-Hydroxybenzoylhydrazine dichloride
-
 Desulfovibrio sp.
4.1.1.61 m-Hydroxybenzoylhydrazine dichloride
-
 Methanospirillum hungatei
4.1.1.61 Sodium dithionite
-
 Desulfovibrio sp.
4.1.1.61 Sodium dithionite
-
 Methanospirillum hungatei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.61 0.004
-
 4-hydroxybenzoate carboxylase with pH-optimum 7.8 Methanospirillum hungatei
4.1.1.61 0.004
-
 4-hydroxybenzoate carboxylase with pH-optimum 7.8 Desulfovibrio sp.
4.1.1.61 0.02
-
 4-hydroxybenzoate carboxylase with pH-optimum 6.4 Methanospirillum hungatei
4.1.1.61 0.02
-
 4-hydroxybenzoate carboxylase with pH-optimum 6.4 Desulfovibrio sp.
4.1.1.61 0.125
-
 phenol
-
 Methanospirillum hungatei
4.1.1.61 0.125
-
 phenol
-
 Desulfovibrio sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.61 membrane loosely associated Methanospirillum hungatei 16020
-
4.1.1.61 membrane loosely associated Desulfovibrio sp. 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.61 K+ phenol carboxylase activity and the two 4-hydroxybenzoate decarboxylase activities are strictly dependent on Methanospirillum hungatei
4.1.1.61 K+ phenol carboxylase activity and the two 4-hydroxybenzoate decarboxylase activities are strictly dependent on Desulfovibrio sp.
4.1.1.61 Mn2+ restores activity of the decarboxylase with pH-optimum 6.4 and of the carboxylase after EDTA treatment, no effect in decarboxylase with pH-optimum 7.8 Methanospirillum hungatei
4.1.1.61 Mn2+ restores activity of the decarboxylase with pH-optimum 6.4 and of the carboxylase after EDTA treatment, no effect in decarboxylase with pH-optimum 7.8 Desulfovibrio sp.
4.1.1.61 Na+ stimulates activity of the enzyme form with pH-optimum pH 7.8 Methanospirillum hungatei
4.1.1.61 Na+ stimulates activity of the enzyme form with pH-optimum pH 7.8 Desulfovibrio sp.
4.1.1.61 NH4+ stimulates activity of the enzyme form with pH-optimum pH 7.8 Methanospirillum hungatei
4.1.1.61 NH4+ stimulates activity of the enzyme form with pH-optimum pH 7.8 Desulfovibrio sp.
4.1.1.61 Rb+ can replace K+ in the phenol carboxylase and partially in both decarboxylases Methanospirillum hungatei
4.1.1.61 Rb+ can replace K+ in the phenol carboxylase and partially in both decarboxylases Desulfovibrio sp.
4.1.1.61 Zn2+ compensates the loss of activity by EDTA Methanospirillum hungatei
4.1.1.61 Zn2+ compensates the loss of activity by EDTA Desulfovibrio sp.

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.61 Desulfovibrio sp.
-
 in an anaerobic consortium with Methanospirillum hungatei, one phenol carboxylase activity and two 4-hydroxybenzoate decarboxylase activities with distinct properties
-
4.1.1.61 Methanospirillum hungatei
-
 in an anaerobic consortium with Desulfovibrio sp., one phenol decarboxylase activity and two 4-hydroxybenzoate decarboxylase activities with distinct properties
-

Storage Stability

EC Number Storage Stability Organism
4.1.1.61 -20°C, gas atmosphere N2/H2, 95%:5%, in potassium phosphate buffer, pH 7.8, stable for months Methanospirillum hungatei
4.1.1.61 -20°C, gas atmosphere N2/H2, 95%:5%, in potassium phosphate buffer, pH 7.8, stable for months Desulfovibrio sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.61 3,4,5-Trihydroxybenzoate
-
 Methanospirillum hungatei Pyrogallol + CO2
-
 ?
4.1.1.61 3,4,5-Trihydroxybenzoate
-
 Desulfovibrio sp. Pyrogallol + CO2
-
 ?
4.1.1.61 3,5-Dihydroxybenzoate
-
 Methanospirillum hungatei 1,3-Dihydroxybenzene + CO2
-
 ?
4.1.1.61 3,5-Dihydroxybenzoate
-
 Desulfovibrio sp. 1,3-Dihydroxybenzene + CO2
-
 ?
4.1.1.61 3,5-Dimethoxy-4-hydroxybenzoate
-
 Methanospirillum hungatei 1,3-Dimethoxy-2-hydroxybenzene + CO2
-
 ?
4.1.1.61 3,5-Dimethoxy-4-hydroxybenzoate
-
 Desulfovibrio sp. 1,3-Dimethoxy-2-hydroxybenzene + CO2
-
 ?
4.1.1.61 3-Chloro-4-hydroxybenzoate
-
 Methanospirillum hungatei 1-Chloro-2-hydroxybenzene + CO2
-
 ?
4.1.1.61 3-Chloro-4-hydroxybenzoate
-
 Desulfovibrio sp. 1-Chloro-2-hydroxybenzene + CO2
-
 ?
4.1.1.61 4-Hydroxybenzoate
-
 Methanospirillum hungatei Phenol + CO2
-
 ?
4.1.1.61 4-Hydroxybenzoate
-
 Desulfovibrio sp. Phenol + CO2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.61 37
-
 enzyme with pH-optimum of pH 7.8 Methanospirillum hungatei
4.1.1.61 37
-
 enzyme with pH-optimum of pH 7.8 Desulfovibrio sp.
4.1.1.61 41
-
 enzyme with pH-optimum of pH 6.4 Methanospirillum hungatei
4.1.1.61 41
-
 enzyme with pH-optimum of pH 6.4 Desulfovibrio sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.61 6.4
-
-
 Methanospirillum hungatei
4.1.1.61 6.4
-
-
 Desulfovibrio sp.
4.1.1.61 6.5
-
 phenol carboxylation Methanospirillum hungatei
4.1.1.61 6.5
-
 phenol carboxylation Desulfovibrio sp.
4.1.1.61 7.8
-
-
 Methanospirillum hungatei
4.1.1.61 7.8
-
-
 Desulfovibrio sp.