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Literature summary extracted from

  • Wakagi, T.; Ohta, T.; Imahori, K.
    Studies on isoleucyl-tRNA synthetase of an extreme thermophile, Thermus thermophilus HB8 (1975), Agric. Biol. Chem., 39, 1573-1580.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.5 K+
-
 Thermus thermophilus
6.1.1.5 Na+
-
 Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.5 0.0001
-
 tRNAIle
-
 Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.5 Mg2+ required Thermus thermophilus
6.1.1.5 Mg2+ optimal concentration is 10 mM Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.5 115000
-
 gel filtration Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.5 Thermus thermophilus
-
 HB8
-
6.1.1.5 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 HB8
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.5 partial Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.5 73.8
-
-
 Thermus thermophilus

Storage Stability

EC Number Storage Stability Organism
6.1.1.5 -20°C, 50% glycerol Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.5 ATP + L-isoleucine + tRNAIle
-
 Thermus thermophilus AMP + diphosphate + L-isoleucyl-tRNAIle
-
 ?
6.1.1.5 ATP + L-isoleucine + tRNAIle
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-isoleucyl-tRNAIle
-
 ?
6.1.1.5 additional information Ile + ATP + enzyme/Ile-AMP-enzyme + diphosphate, isoleucine-dependent ATP-diphosphate exchange Thermus thermophilus ?
-
 ?
6.1.1.5 additional information Ile + ATP + enzyme/Ile-AMP-enzyme + diphosphate, isoleucine-dependent ATP-diphosphate exchange Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.5 62
-
 isoleucine-tRNA formation with E. coli tRNA Thermus thermophilus
6.1.1.5 75
-
 isoleucine tRNA formation with Thermus thermophilus tRNA Thermus thermophilus
6.1.1.5 80
-
 isoleucine dependent ATP-diphosphate exchange Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.1.1.5 77
-
 half-life is 15 min, inactivation is completely suppressed by addition of either E. coli or Thermus thermophilus tRNA Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.5 7.7
-
-
 Thermus thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.1.1.5 6 8.8 about 30% of maximal activity at pH 6.0 and 8.8 Thermus thermophilus