EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.1.5 | overexpression in Escherichia coli | Lactococcus lactis |
EC Number | General Stability | Organism |
---|---|---|
4.1.1.5 | very unstable, especially when diluted, at 0.05 mg/ml the activity is totally lost within 24 h at 4°C. Addition of bovine serum albumin, 5 mg/ml, allows the preservation of the activity for at least 2 weeks | Lactococcus lactis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.5 | EDTA | 2 mM Mn2+ restores 82% of the activity, 2 mM Zn2+ restores 69% of the initial activity | Lactococcus lactis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.5 | 10 | - |
2-Hydroxy-2-methyl-3-oxobutanoate | in presence of Leu | Lactococcus lactis | |
4.1.1.5 | 75 | - |
2-Hydroxy-2-methyl-3-oxobutanoate | in absence of Leu | Lactococcus lactis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.1.5 | 26500 | - |
6 * 26500, SDS-PAGE | Lactococcus lactis |
4.1.1.5 | 150000 | - |
gel filtration | Lactococcus lactis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.5 | 2-Hydroxy-2-methyl-3-oxobutanoate | Lactococcus lactis | activation by Leu, Val and Ile | ? | - |
? | |
4.1.1.5 | 2-Hydroxy-2-methyl-3-oxobutanoate | Lactococcus lactis NCDO 2118 | activation by Leu, Val and Ile | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.5 | Lactococcus lactis | - |
- |
- |
4.1.1.5 | Lactococcus lactis | - |
subsp. lactis | - |
4.1.1.5 | Lactococcus lactis NCDO 2118 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.5 | - |
Lactococcus lactis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.1.5 | 454.3 | - |
- |
Lactococcus lactis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.5 | 2-Hydroxy-2-methyl-3-oxobutanoate | - |
Lactococcus lactis | (R)-3-Hydroxybutan-2-one + CO2 | - |
? | |
4.1.1.5 | 2-Hydroxy-2-methyl-3-oxobutanoate | - |
Lactococcus lactis NCDO 2118 | (R)-3-Hydroxybutan-2-one + CO2 | - |
? | |
4.1.1.5 | 2-Hydroxy-2-methyl-3-oxobutanoate | activation by Leu, Val and Ile | Lactococcus lactis | ? | - |
? | |
4.1.1.5 | 2-Hydroxy-2-methyl-3-oxobutanoate | activation by Leu, Val and Ile | Lactococcus lactis NCDO 2118 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.1.5 | hexamer | 6 * 26500, SDS-PAGE | Lactococcus lactis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.5 | 6 | - |
- |
Lactococcus lactis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.5 | 5.4 | 6.9 | 75% of maximal activity at pH 5.4 and at pH 6.9 | Lactococcus lactis |