Literature summary extracted from

Eberhard, M.; Kirschner, K.
Modification of a catalytically important residues of indoleglycerol-phosphate synthase from Escherichia coli (1989), FEBS Lett., 245, 219-222.

Protein Variants

EC Number	Protein Variants	Comment	Organism
4.1.1.48	L55S	Lys55Ser with 60fold decrease in turnover-number and 450fold increase in Km-value	Escherichia coli

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.1.1.48	0.0012	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate	wild-type enzyme	Escherichia coli
4.1.1.48	0.54	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate	mutant Lys55Ser	Escherichia coli

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.1.1.48	Escherichia coli	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.1.1.48	1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate	-	Escherichia coli	1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O	-	?

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4.1.1.48	0.12	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate	mutant enzyme Lys55Ser	Escherichia coli
4.1.1.48	7.2	-	1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate	wild-type enzyme	Escherichia coli

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Release 2023.1 (January 2023)