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Literature summary extracted from

  • Eberhard, M.; Tsai-Pflugfelder, M.; Bolewska, K.; Hommel, U.; Kirschner, K.
    Indoleglycerol phosphate synthase - phosphoribosyl anthranilate isomerase: comparison of the bifunctional enzyme from Escherichia coli with engineered monofunctional domains (1995), Biochemistry, 34, 5419-5428.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.24 separation of the 2 domains of the indole-3-glycerol-phosphate synthetase/PRA isomerase complex on the gene level, expression of monofunctional PRAI[ML256-452] in Escherichia coli W3110 trpR, deltatrp EA2 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.24
-
 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.48 additional information engineering of a monofunctional enzyme by separating the domains on the gene level and expression as monofunctional enzymes Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.48 Reduced 1-[2-carboxyphenyl)amino]-1-deoxyribulose 5-phosphate
-
 Escherichia coli
5.3.1.24 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate product inhibition Escherichia coli
5.3.1.24 additional information not inhibited by indoleglycerol phosphate Escherichia coli
5.3.1.24 reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate competitive inhibitor Escherichia coli
5.3.1.24 reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate competitive inhibitor Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.48 0.000047
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate genetically engineered monofunctional enzyme form IGPS[1-259] Escherichia coli
4.1.1.48 0.00042
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate bifunctional enzyme Escherichia coli
4.1.1.48 0.013
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate genetically engineered monofunctional enzyme form IGPS[1-252FAG] Escherichia coli
5.3.1.24 0.0047
-
 N-(5-phospho-beta-D-ribosyl)anthranilate 25°C, monomeric domain PRAI[ML256-452] Escherichia coli
5.3.1.24 0.0049
-
 N-(5-phospho-beta-D-ribosyl)anthranilate 25°C, bifunctional IGPS:PRAI Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.24 23000
-
-
 Saccharomyces cerevisiae
5.3.1.24 23000
-
 monomeric domain PRAI[ML256-452], gel filtration and analytical ultracentrifugation Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Escherichia coli involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Saccharomyces cerevisiae involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate Saccharomyces cerevisiae xPRAI involved in tryptophan biosynthesis from chorismate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.48 Escherichia coli
-
 bifunctional enzyme phosphoribosylanthranilate isomerase-indoleglycerol phosphate synthetase, Ec4.1.1.48/Ec5.3.1.24
-
5.3.1.24 Escherichia coli
-
 bifunctional indole-3-glycerol-phosphate synthetase/PRA isomerase complex
-
5.3.1.24 Saccharomyces cerevisiae
-
 xPRAI
-
5.3.1.24 Saccharomyces cerevisiae xPRAI
-
 xPRAI
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.48 monofunctional enzyme Escherichia coli
5.3.1.24 monomeric domain PRAI[ML256-452] and bifunctional IGPS:PRAI Escherichia coli
5.3.1.24 recombinant xPRAI, expressed in Escherichia coli Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.24 98.7
-
 monofunctional domain PRAI[ML256-452] Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.48 1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate
-
 Escherichia coli 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O
-
 ?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate
-
 Escherichia coli 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate
-
 Saccharomyces cerevisiae 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Escherichia coli 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Saccharomyces cerevisiae 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate
-
 Saccharomyces cerevisiae xPRAI 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?
5.3.1.24 N-(5-phospho-beta-D-ribosyl)anthranilate involved in tryptophan biosynthesis from chorismate Saccharomyces cerevisiae xPRAI 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.1.24 PRAI[ML256-452] engineered monofunctional variant of IGPS:PRAI, excised monomeric domain Escherichia coli
5.3.1.24 PRAI[ML256-452] engineered monofunctional variant of IGPS:PRAI, excised monomeric domain Saccharomyces cerevisiae
5.3.1.24 xPRAI monomeric variant of PRAI from Saccharomyces cerevisiae expressed in Escherichia coli Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.48 3.2
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate genetically engineered monofunctional enzyme form IGPS[1-259] Escherichia coli
4.1.1.48 3.6
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate bifunctional enzyme Escherichia coli
4.1.1.48 4.3
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate genetically engineered monofunctional enzyme form IGPS[1-252FAG] Escherichia coli
5.3.1.24 32
-
 N-(5-phospho-beta-D-ribosyl)anthranilate 25°C, monomeric domain PRAI[ML256-452] Escherichia coli
5.3.1.24 40
-
 N-(5-phospho-beta-D-ribosyl)anthranilate 25°C Escherichia coli
5.3.1.24 40
-
 N-(5-phospho-beta-D-ribosyl)anthranilate bifunctional IGPS:PRAI Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.3.1.24 0.0022
-
 reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate recombinant xPRAI, expressed in Escherichia coli Saccharomyces cerevisiae
5.3.1.24 0.0065
-
 reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate 25°C, bifunctional IGPS:PRAI Escherichia coli
5.3.1.24 0.0068
-
 reduced 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate 25°C, monomeric domain PRAI[ML256-452] Escherichia coli