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Literature summary extracted from

  • Grosjean, H.; Charlier, J.
    Comparative studies on the isoleucyl-tRNA synthetases from Bacillus stearothermophilus and Escherichia coli. II. The effect of magnesium ions in the transacylation reaction (1971), FEBS Lett., 18, 342-346.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.5 Mg2+ optimal concentration is 5-6 mM Geobacillus stearothermophilus
6.1.1.5 Mg2+ optimum at very low concentration (0.5-0.7 mM), slight inhibition at higher concentration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.5 Escherichia coli
-
-
-
6.1.1.5 Geobacillus stearothermophilus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.5 ATP + L-isoleucine + tRNAIle
-
Escherichia coli AMP + diphosphate + L-isoleucyl-tRNAIle
-
?
6.1.1.5 ATP + L-isoleucine + tRNAIle
-
Geobacillus stearothermophilus AMP + diphosphate + L-isoleucyl-tRNAIle
-
?